Department of Food Science and Technology, Chung-Ang University, Ansung, Korea.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2009 Apr;26(4):518-26. doi: 10.1080/02652030802562904.
The molecular structures of enniatins H, I, and MK1688 and beauvericin were investigated by liquid chromatography-tandem mass spectrometry (LC-MS/MS). MS fragmentation occurred by loss of -CO after opening of the cyclic molecule to carbonyl carbon, and cleavage of the peptide and ester bonds in the molecular structure. Fusarium oxysporum KFCC 11363P was tested for its ability to produce beauvericin and enniatins H, I, and MK1688 on five cereal substrates: rice, barley, maize, wheat, and Indian millet kernels. Furthermore, optimal conditions for the production of the four mycotoxins by the Fusarium isolate were examined on maize at four temperatures (15, 20, 25, and 30 degrees C) and at three moisture contents (10, 20, and 40%). Large amounts of beauvericin and enniatin H were present in maize cultures at 25 degrees C (232.4 and 196.4 microg g(-1), respectively). Enniatins I and MK1688 were maximally formed at 20 degrees C (221.5 and 180.2 microg g(-1), respectively). The optimal moisture contents for the production of enniatins H (196.4 microg g(-1)) and MK1688 (165.6 microg g(-1)), were 40%.
通过液相色谱-串联质谱法（LC-MS/MS）研究了恩镰孢菌素 H、I 和 MK1688 以及 beauvericin 的分子结构。通过打开环状分子至羰基碳，失去-CO 以及分子结构中肽和酯键的断裂，发生了 MS 碎裂。测试了尖孢镰刀菌 KFCC 11363P 在五种谷物基质（大米、大麦、玉米、小麦和印度小米）上产生 beauvericin 和恩镰孢菌素 H、I 和 MK1688 的能力。此外，还在玉米上研究了该镰刀菌分离物在四个温度（15、20、25 和 30 摄氏度）和三个水分含量（10、20 和 40%）下产生这四种真菌毒素的最佳条件。在 25 摄氏度时，玉米培养物中存在大量的 beauvericin 和恩镰孢菌素 H（分别为 232.4 和 196.4 µg/g）。恩镰孢菌素 I 和 MK1688 在 20 摄氏度时形成最多（分别为 221.5 和 180.2 µg/g）。产生恩镰孢菌素 H（196.4 µg/g）和 MK1688（165.6 µg/g）的最佳水分含量为 40%。
