Department of Food Science and Technology, BET Research Institute, Chung-Ang University, Anseong 456-756, Republic of Korea.
J Biosci Bioeng. 2011 Mar;111(3):279-85. doi: 10.1016/j.jbiosc.2010.10.013. Epub 2010 Nov 20.
The aim of this study was to optimize the culture conditions for the production of biological cyclic hexadepsipeptides (enniatins H, I and MK1688) from Fusarium oxysporum KFCC 11363P. Tests of 10 complete or chemically defined liquid culture media revealed that Fusarium defined medium was the best for the production of enniatins (produced amounts: enniatin H, 185.4 mg/L; enniatin I, 349.1mg/L; enniatin MK1688, 541.1mg/L; and total enniatins, 1075.6 mg/L). On the eighth day after inoculation, the maximal production of enniatins was observed at 25°C in Fusarium defined medium. The optimal carbon and nitrogen sources for producing biological cyclic hexadepsipeptides (enniatins H, I, and MK1688) were sucrose and NaNO(3), respectively, and their optimal concentrations were determined by the principle of response surface methodology. It was confirmed that using the optimized growth medium compositions increased the amounts of enniatins H, I, and MK1688, and total enniatins produced to 695.2, 882.4, 824.8, and 2398.5mg/L, respectively. These findings will assist in formulating microbiological media useful for enniatin research.
本研究旨在优化生物环状六肽（恩镰菌素 H、I 和 MK1688）的发酵条件，所用菌株为尖孢镰刀菌 KFCC 11363P。通过对 10 种完全或化学限定液体培养基的测试发现，尖孢镰刀菌限定培养基最有利于恩镰菌素的产生（产生量：恩镰菌素 H，185.4mg/L；恩镰菌素 I，349.1mg/L；恩镰菌素 MK1688，541.1mg/L；总恩镰菌素，1075.6mg/L）。在接种后第 8 天，在 25°C 时，尖孢镰刀菌限定培养基中恩镰菌素的产量达到最大。对于生物环状六肽（恩镰菌素 H、I 和 MK1688）的产生，最佳碳源和氮源分别为蔗糖和 NaNO3，其最佳浓度通过响应面法的原理确定。结果证实，使用优化的生长培养基组成可使恩镰菌素 H、I、MK1688 和总恩镰菌素的产量分别提高到 695.2、882.4、824.8 和 2398.5mg/L。这些发现将有助于制定用于恩镰菌素研究的微生物培养基。
