Lee Hee-Seok, Song Hyuk-Hwan, Jeong Jin-Ho, Shin Cha-Gyun, Choi Sang-Un, Lee Chan
Department of Food Science and Technology, BET Research Institute, Chung-Ang University, Ansung 456-756, Republic of Korea.
Toxicon. 2008 Jun 1;51(7):1178-85. doi: 10.1016/j.toxicon.2008.02.002. Epub 2008 Feb 19.
Enniatins (ENs) H, I, and MK1688 and beauvericin (BEA) were purified from concentrated chloroform extracts of Fusarium oxysporum KFCC 11363P submerged cultures using HPLC, and their in vitro cytotoxicities were evaluated against four human carcinoma cell lines (lung, A549; ovarian, SK-OV-3; skin melanoma, SK-MEL-2; and colon, HCT15) using the sulforhodamine B (SRB) method. ENs I and MK1688 inhibited the growth of cancer cell lines most strongly and had similar cytotoxic effects on the tested human cancer cell cultures. The cytotoxicity of ENs I and MK1688 was three- to fourfold higher than that of BEA and EN H. When cultivated in Fusarium-defined medium (FDM), the concentrations of ENs and BEA produced in F. oxysporum KFCC 11363P decreased in the following order: EN MK1688 (0.81 g/L) >EN I (0.55 g/L) >BEA (0.17 g/L) > EN H (0.16 g/L). This study has shown that ENs H, I, and MK1688 exhibit cytotoxicity against certain adenocarcinoma cell lines. The results indicate the need for more investigations into the significance of the biological properties of these new ENs.
从尖孢镰刀菌KFCC 11363P液体培养物的浓缩氯仿提取物中，通过高效液相色谱法纯化恩镰孢菌素（ENs）H、I、MK1688和白僵菌素（BEA），并采用磺酰罗丹明B（SRB）法评估它们对四种人类癌细胞系（肺癌A549、卵巢癌SK-OV-3、皮肤黑色素瘤SK-MEL-2和结肠癌HCT15）的体外细胞毒性。ENs I和MK1688对癌细胞系生长的抑制作用最强，对测试的人类癌细胞培养物具有相似的细胞毒性作用。ENs I和MK1688的细胞毒性比BEA和EN H高3至4倍。在镰刀菌限定培养基（FDM）中培养时，尖孢镰刀菌KFCC 11363P产生的ENs和BEA浓度按以下顺序降低：EN MK1688（0.81 g/L）>EN I（0.55 g/L）>BEA（0.17 g/L）>EN H（0.16 g/L）。本研究表明，ENs H、I和MK1688对某些腺癌细胞系具有细胞毒性。结果表明需要对这些新的ENs生物学特性的重要性进行更多研究。
