Takahashi Hajime, Miya Satoko, Igarashi Kazunori, Suda Takayuki, Kuramoto Shintaro, Kimura Bon
Department of Food Science and Technology, Faculty of Marine Science, Tokyo University of Marine Science and Technology, Minato-ku, Tokyo 108-8477, Japan.
J Food Prot. 2009 Jul;72(7):1476-80. doi: 10.4315/0362-028x-72.7.1476.
Listeria monocytogenes is of great concern as a foodborne pathogen. Many ready-to-eat foods are widely contaminated with this organism and have caused listeriosis outbreaks and sporadic cases in many countries. In Japan, there is a high incidence of L. monocytogenes contamination, specifically in raw ready-to-eat seafood. Identical L. monocytogenes subtypes have been isolated repeatedly from samples of food manufactured at a given store or processing plant, and researchers suspected that certain L. monocytogenes isolates have formed biofilms at these sites. A microtiter plate biofilm formation assay was conducted, and all raw ready-to-eat seafood isolates tested were able to form biofilms to various degrees. Biofilm formation by L. monocytogenes isolates of lineage I was significantly greater (P = 0.000) than that by isolates of lineage II. However, isolates of clonal lineages formed different levels of biofilms, indicating that the ability to form a biofilm is affected positively or negatively by environmental factors.
单核细胞增生李斯特菌作为一种食源性病原体备受关注。许多即食食品被这种微生物广泛污染，并在许多国家引发了李斯特菌病疫情和散发病例。在日本，单核细胞增生李斯特菌的污染发生率很高，尤其是在即食生鲜海产品中。从特定商店或加工厂生产的食品样本中多次分离出相同的单核细胞增生李斯特菌亚型，研究人员怀疑某些单核细胞增生李斯特菌菌株已在这些场所形成生物膜。进行了微量滴定板生物膜形成试验，所有测试的即食生鲜海产品分离株均能不同程度地形成生物膜。I 系单核细胞增生李斯特菌分离株形成生物膜的能力显著高于（P = 0.000）II 系分离株。然而，克隆谱系的分离株形成生物膜的水平不同，这表明形成生物膜的能力受到环境因素的正向或负向影响。
