Centre for Geographic Medicine Research (Coast), Kenya Medical Research Institute/Wellcome Trust Research Programme, Kilifi, Kenya.
Transfusion. 2009 Dec;49(12):2594-8. doi: 10.1111/j.1537-2995.2009.02344.x. Epub 2009 Aug 4.
Hospitalized children in sub-Saharan Africa frequently receive whole blood transfusions for severe anemia. The risk from bacterial contamination of blood for transfusion in sub-Saharan Africa is not known. This study assessed the frequency of bacterial contamination of pediatric whole blood transfusions at a referral hospital in Kenya.
This was an observational study. Over the course of 1 year, bacteriologic cultures were performed on 434 of the 799 blood packs issued to children by the blood bank of Coast Provincial General Hospital, Mombasa. Clinical outcome was not assessed.
Forty-four bacterial contaminants were isolated from 38 blood packs-an overall contamination frequency of 8.8% (95% confidence interval, 6.1%-11.4%). Sixty-four percent of the bacteria isolated were Gram-negative. Many of the isolates are usually found in the environment and the most likely source of contamination was considered to be the hospital blood bank.
Bacterial contamination of whole blood may be a significant but unrecognized hazard of blood transfusion for children in sub-Saharan Africa. Further work is needed to clarify the extent of the problem and its clinical consequences. Increased awareness and adherence to basic principles of asepsis in the hospital blood bank may be important immediate interventions.
撒哈拉以南非洲的住院儿童经常因严重贫血接受全血输血。在撒哈拉以南非洲，用于输血的血液受到细菌污染的风险尚不清楚。本研究评估了肯尼亚一家转诊医院儿科全血输血中细菌污染的频率。
这是一项观察性研究。在一年的时间里，对莫巴萨省沿海总医院血库发放给儿童的 799 个血袋中的 434 个进行了细菌培养。未评估临床结果。
从 38 个血袋中分离出 44 个细菌污染物-总体污染频率为 8.8%（95%置信区间，6.1%-11.4%）。分离出的细菌中 64%为革兰氏阴性菌。许多分离物通常存在于环境中，污染的最可能来源被认为是医院血库。
全血的细菌污染可能是撒哈拉以南非洲儿童输血的一个重大但未被认识到的危险。需要进一步的工作来阐明问题的程度及其临床后果。提高医院血库中无菌操作的认识并遵守基本的无菌原则可能是重要的即时干预措施。
