Nicholas A P, Hancock M B
Department of Anatomy and Neurosciences, University of Texas Medical Branch, Galveston 77550.
Neurosci Lett. 1990 Jan 1;108(1-2):22-8. doi: 10.1016/0304-3940(90)90700-j.
Following the iontophoretic deposition of Phaseolus vulgaris leucoagglutinin (PHA-L) into the rostral medullary raphe, which included portions of the caudal nucleus raphe magnus, rostral nucleus raphe pallidus, rostral nucleus raphe obscurus and rostral nucleus reticularis paragigantocellularis, two-color immunoperoxidase staining was employed to demonstrate contiguity between PHA-L-immunoreactive (PHA-LI) varicose fibers and boutons and medullary catecholamine (CA) cells. Raphe projections were contiguous with phenylethanolamine N-methyltransferase-immunoreactive (PNMTI) neurons in the C1, C2 and C3 cell groups and with tyrosine hydroxylase-immunoreactive (THI) neurons in the A1 and A2 cell groups. Contiguity between PHA-LI processes and medullary CA cells was observed most frequently in the C1 cell group. Preliminary findings of this study have been presented previously.
将菜豆白细胞凝集素（PHA-L）经离子电渗法注入延髓头端中缝，其中包括尾侧中缝大核、头侧中缝苍白核、头侧中缝隐核和头侧网状旁巨细胞网状核的部分区域，随后采用双色免疫过氧化物酶染色来显示PHA-L免疫反应性（PHA-LI）曲张纤维和终扣与延髓儿茶酚胺（CA）细胞之间的连续性。中缝投射与C1、C2和C3细胞群中苯乙醇胺N-甲基转移酶免疫反应性（PNMTI）神经元以及A1和A2细胞群中酪氨酸羟化酶免疫反应性（THI）神经元相邻。在C1细胞群中，PHA-LI突起与延髓CA细胞之间的连续性最为常见。本研究的初步结果已在之前发表。
