Luppi P H, Fort P, Kitahama K, Denoroy L, Jouvet M
Département de Médecine Expérimentale, INSERM U52, CNRS UA1195, Faculté de Médecine, Université Claude Bernard, Lyon, France.
Neurosci Lett. 1989 Nov 20;106(1-2):29-35. doi: 10.1016/0304-3940(89)90197-3.
By means of a double immunostaining technique using unconjugated cholera-toxin B subunit (CTb) as a retrograde tracer combined with phenylethanolamine-N-methyltransferase (PNMT) immunohistochemistry, we demonstrated that the nucleus raphe pallidus of the cat receives a major projection from the ventrolateral part of the rostral medulla corresponding to the nucleus paragigantocellularis lateralis and the ventrolateral medullary reticular formation just caudal to it. We further showed that nearly 60% of the total CTb-labeled cells in this region are immunoreactive to PNMT. These double-labeled cells constitute one-third of the total PNMT-immunoreactive cells.
通过使用未结合的霍乱毒素B亚基（CTb）作为逆行示踪剂的双重免疫染色技术，并结合苯乙醇胺-N-甲基转移酶（PNMT）免疫组织化学，我们证明猫的中缝苍白核接受来自延髓前部腹外侧部分的主要投射，该部分对应于外侧巨细胞旁核及其尾侧的腹外侧髓质网状结构。我们进一步表明，该区域中CTb标记细胞总数的近60%对PNMT具有免疫反应性。这些双标记细胞占PNMT免疫反应性细胞总数的三分之一。
