De Jong Laetitia, Moreau Xavier, Dalia Jocelyne, Coustau Christine, Thiéry Alain
UMR-CNRS 6116 IMEP (Institut Méditerranéen d'Ecologie et de Paléoécologie), Université de Provence, Equipe Biomarqueurs & Bioindicateurs Environnementaux, case 97, 13331 Marseille Cedex 3, France.
Acta Trop. 2009 Dec;112(3):266-9. doi: 10.1016/j.actatropica.2009.08.008. Epub 2009 Aug 13.
The Asian tiger mosquito Aedes albopictus, vector of various human viruses and parasites, has recently spread and established in many temperate regions including European countries. In the present study, we developed a simple PCR-based assay (the amplification of the internal transcribed spacer ITS2 within nuclear ribosomal rDNA) for molecular identification of A. albopictus and confirmed its presence in Corsica island. This assay may (i) facilitate future large scale studies and avoid misidentifications, especially because of the presence of co-occurring close species in this island and (ii) contribute to the monitoring of A. albopictus populations required for targeted control programs.
亚洲虎蚊白纹伊蚊是多种人类病毒和寄生虫的传播媒介，最近已扩散并在包括欧洲国家在内的许多温带地区定殖。在本研究中，我们开发了一种基于PCR的简单检测方法（扩增核糖体核糖核酸rDNA中的内转录间隔区ITS2）用于白纹伊蚊的分子鉴定，并证实其在科西嘉岛存在。该检测方法可能（i）有助于未来的大规模研究并避免错误鉴定，特别是由于该岛存在同时出现的近缘物种；（ii）有助于监测针对性控制计划所需的白纹伊蚊种群。
