Department of Vector Ecology and Environment, Institute of Tropical Medicicine, Nagasaki University, Nagasaki 852-8523, Japan.
Jpn J Infect Dis. 2010 Sep;63(5):312-6.
Internal transcribed spacer regions of ribosomal DNA were sequenced, and new species-specific primers were designed to simplify the molecular identification of five morphologically related subgenus Stegomyia mosquito species--Aedes aegypti, Ae. albopictus, Ae. riversi, Ae. flavopictus, and Ae. daitensis--found in the Ryukyu Archipelago, Japan. Each newly designed primer was able to amplify a species-specific fragment with a different size. Conditions for multiplex PCR were optimized to identify all five species in a single PCR. This method is a convenient tool for entomological field surveys, particularly in arbovirus endemic/epidemic areas where some of these species coexist.
核糖体 DNA 的内部转录间隔区进行了测序，并设计了新的种特异性引物，以简化对在日本琉球群岛发现的 5 种形态相关的 Stegomyia 蚊亚属（埃及伊蚊、白纹伊蚊、溪流伊蚊、埃及斑蚊和日本脑炎蚊）的分子鉴定。每个新设计的引物都能够扩增出具有不同大小的种特异性片段。优化了多重 PCR 的条件，以在单个 PCR 中鉴定所有 5 个物种。这种方法是昆虫学野外调查的便捷工具，特别是在存在这些物种共存的虫媒病毒流行/疫区。
