Ji Pei-Feng, Yao Cui-Luan, Wang Zhi-Yong
Key Laboratory of Science and Technology for Aquaculture and Food Safety of Fujian Province University, Fisheries college/Fisheries Biotechnology Institute, Jimei University, Xiamen 361021, China.
Fish Shellfish Immunol. 2009 Oct;27(4):563-70. doi: 10.1016/j.fsi.2009.08.001. Epub 2009 Aug 14.
The effects of some pathogen-associated molecular patterns (PAMPs) (laminarin, LPS and poly I:C) on total hemocyte counts (THC), phenoloxidase (PO) activity, superoxide anion production and lectin, prophenoloxidase, lysozyme, cytosolic manganese superoxide dismutase (C-MnSOD) and catalase (CAT) gene expression were studied. The results showed that the production or activity of most tested immune factors and the expression of most tested genes were up-regulated after stimulation with PAMPs, among which the highest value of lectin with 4.4 times as much as that of the control group appeared at 6 h in hemocytes, of CAT with 47 times as much as that of the control group appeared at 12 h in hepatopancreas, and with 2.7 times higher than that of the control group at 24 h of C-MnSOD in hepatopancreas after laminarin injection. The peak value of proPO, lysozyme and C-MnSOD appeared at 6 h in hepatopancreas, 24 h in hepatopancreas and 24 h in hemocytes after LPS injection, respectively. The highest expression level of lysozyme appeared at 12 h in hemocytes after poly I:C injection. However, significant decreases of PO activity in hemocytes and lectin expression in hepatopancreas were found after poly I:C injection, and a dramatic down-regulation of proPO expression from 3 h to 48 h was found in hemocytes after injection with laminarin, LPS and poly I:C. The results suggest that the shrimp immune response could be activated or inhibited by different PAMPs, and that the hepatopancreas also plays a key role by synthesizing immune factors.
研究了一些病原体相关分子模式（PAMPs）（海带多糖、脂多糖和聚肌苷酸胞苷酸）对总血细胞计数（THC）、酚氧化酶（PO）活性、超氧阴离子产生以及凝集素、前酚氧化酶、溶菌酶、胞质锰超氧化物歧化酶（C-MnSOD）和过氧化氢酶（CAT）基因表达的影响。结果表明，在用PAMPs刺激后，大多数检测的免疫因子的产生或活性以及大多数检测基因的表达均上调，其中凝集素在血细胞中于6小时达到最高值，是对照组的4.4倍；CAT在肝胰腺中于12小时达到最高值，是对照组的47倍；海带多糖注射后，肝胰腺中的C-MnSOD在24小时比对照组高2.7倍。脂多糖注射后，前酚氧化酶、溶菌酶和C-MnSOD的峰值分别出现在肝胰腺的6小时、肝胰腺的24小时和血细胞的24小时。聚肌苷酸胞苷酸注射后，溶菌酶在血细胞中的最高表达水平出现在12小时。然而，聚肌苷酸胞苷酸注射后，血细胞中的PO活性和肝胰腺中的凝集素表达显著降低，注射海带多糖、脂多糖和聚肌苷酸胞苷酸后，血细胞中前酚氧化酶的表达在3小时至48小时显著下调。结果表明，不同的PAMPs可激活或抑制对虾的免疫反应，并且肝胰腺通过合成免疫因子也发挥关键作用。
