Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, 2 Darwin Street, Cantoblanco, 28049 Madrid, Spain.
Water Res. 2010 Jan;44(2):427-38. doi: 10.1016/j.watres.2009.07.026. Epub 2009 Jul 25.
Pharmaceuticals in the aquatic environment do not appear singly and usually occur as complex mixtures, whose combined effect may exhibit toxicity to the aquatic biota. We report an environmental application of the combination index (CI)-isobologram equation, a method widely used in pharmacology to study drug interactions, to determine the nature of toxicological interactions of three fibrates toward two aquatic bioluminescent organisms, Vibrio fischeri and the self-luminescent cyanobacterial recombinant strain Anabaena CPB4337. The combination index-isobologram equation method allows computerized quantitation of synergism, additive effect and antagonism. In the Vibrio test, the fibrate combinations showed antagonism at low effect levels that turned into an additive effect or synergism at higher effect levels; by contrast, in the Anabaena test, the fibrate combinations showed a strong synergism at the lowest effect levels and a very strong antagonism at high effect levels. We also evaluated the nature of the interactions of the three fibrates with a real wastewater sample in the cyanobacterial test. We propose that the combination index-isobologram equation method can serve as a useful tool in ecotoxicological assessment.
水环境中的药物并非单独存在，通常以复杂混合物的形式存在，其综合效应可能对水生生物群具有毒性。我们报告了组合指数（CI）-等效应线方程的环境应用，该方法在药理学中广泛用于研究药物相互作用，以确定三种纤维酸酯对两种水生发光生物，发光弧菌和自发光蓝藻重组菌株鱼腥藻 CPB4337 的毒性相互作用的性质。组合指数-等效应线方程方法允许计算机化定量协同作用、相加作用和拮抗作用。在发光弧菌测试中，纤维酸酯组合在低效应水平下表现出拮抗作用，而在更高的效应水平下则表现出相加作用或协同作用；相比之下，在鱼腥藻测试中，纤维酸酯组合在最低效应水平下表现出强烈的协同作用，在高效应水平下表现出非常强烈的拮抗作用。我们还在蓝藻测试中评估了三种纤维酸酯与实际废水样品的相互作用性质。我们提出，组合指数-等效应线方程方法可以作为生态毒理学评估的有用工具。
