SOSS复合物参与基因组稳定性的维持。
SOSS complexes participate in the maintenance of genomic stability.
作者信息
Huang Jun, Gong Zihua, Ghosal Gargi, Chen Junjie
机构信息
Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, CT 06520, USA.
出版信息
Mol Cell. 2009 Aug 14;35(3):384-93. doi: 10.1016/j.molcel.2009.06.011.
Abstract
Proteins that bind to single-stranded DNA (ssDNA) are essential for DNA replication, recombinational repair, and maintenance of genomic stability. Here, we describe the characterization of an ssDNA-binding heterotrimeric complex, SOSS (sensor of ssDNA) in human, which consists of human SSB homologs hSSB1/2 (SOSS-B1/2) and INTS3 (SOSS-A) and a previously uncharacterized protein C9orf80 (SOSS-C). We have shown that SOSS-A serves as a central adaptor required not only for SOSS complex assembly and stability, but also for facilitating the accumulation of SOSS complex to DNA ends. Moreover, SOSS-depleted cells display increased ionizing radiation sensitivity, defective G2/M checkpoint, and impaired homologous recombination repair. Thus, our study defines a pathway involving the sensing of ssDNA by SOSS complex and suggests that this SOSS complex is likely involved in the maintenance of genome stability.
摘要
与单链DNA（ssDNA）结合的蛋白质对于DNA复制、重组修复以及基因组稳定性的维持至关重要。在此，我们描述了一种人类中的ssDNA结合异源三聚体复合物SOSS（单链DNA传感器）的特性，它由人类SSB同源物hSSB1/2（SOSS-B1/2）和INTS3（SOSS-A）以及一种此前未被表征的蛋白质C9orf80（SOSS-C）组成。我们已经表明，SOSS-A不仅是SOSS复合物组装和稳定性所需的核心衔接蛋白，而且还促进SOSS复合物在DNA末端的积累。此外，SOSS缺失的细胞表现出电离辐射敏感性增加、G2/M检查点缺陷以及同源重组修复受损。因此，我们的研究定义了一条涉及SOSS复合物感知ssDNA的途径，并表明该SOSS复合物可能参与基因组稳定性的维持。
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Cell. 2008 Oct 17;135(2):250-60. doi: 10.1016/j.cell.2008.09.054.
2
Mre11 dimers coordinate DNA end bridging and nuclease processing in double-strand-break repair.Mre11二聚体在双链断裂修复中协调DNA末端桥接和核酸酶加工。
Cell. 2008 Oct 3;135(1):97-109. doi: 10.1016/j.cell.2008.08.017.
3
Mre11 nuclease activity has essential roles in DNA repair and genomic stability distinct from ATM activation.Mre11核酸酶活性在DNA修复和基因组稳定性中具有不同于ATM激活的重要作用。
Cell. 2008 Oct 3;135(1):85-96. doi: 10.1016/j.cell.2008.08.015.
4
Sae2, Exo1 and Sgs1 collaborate in DNA double-strand break processing.Sae2、Exo1和Sgs1在DNA双链断裂处理过程中协同作用。
Nature. 2008 Oct 9;455(7214):770-4. doi: 10.1038/nature07312. Epub 2008 Sep 21.
5
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6
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9
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10
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