Human Performance Laboratory, Department of Kinesiology, University of Connecticut, Storrs, CT 06269, USA.
Steroids. 2009 Nov-Dec;74(13-14):1033-9. doi: 10.1016/j.steroids.2009.08.002. Epub 2009 Aug 14.
The purpose of this study was to examine the acute effect of resistance exercise (RE) on muscle androgen receptor (AR) and glucocorticoid receptor (GR) protein content. Fifteen resistance-trained men (n=8; 21+/-1 years, 175.3+/-6.7 cm, 90.8+/-11.6 kg) and women (n=7; 24+/-5 years, 164.6+/-6.7 cm, 76.4+/-15.6 kg) completed 6 sets of 10 repetitions of heavy squats. Blood samples were obtained before RE, after 3 and 6 sets of squats, and 5, 15, 30 and 70 min after RE. Muscle biopsies from the vastus lateralis were obtained before RE, and 10 min and 70 min after RE. Blood samples were analyzed for total and free testosterone concentrations and muscle samples were analyzed for AR and GR protein content. Circulating total testosterone increased significantly (p< or =0.05) in men and free testosterone increased in men and women with exercise. AR was significantly reduced at 70 min post-exercise in men and at 10 min post-exercise in women compared to pre-exercise. There were no changes in GR following RE, but GR was significantly higher in women compared to men. These findings support a current paradigm for stabilization followed by a reduction and then a rebound in the acute AR response to RE but demonstrate that gender differences exist in the timeline of the AR response.
本研究旨在探究抗阻运动（RE）对肌肉雄激素受体（AR）和糖皮质激素受体（GR）蛋白含量的急性影响。15 名抗阻训练的男性（n=8；21+/-1 岁，175.3+/-6.7cm，90.8+/-11.6kg）和女性（n=7；24+/-5 岁，164.6+/-6.7cm，76.4+/-15.6kg）完成了 6 组 10 次的大重量深蹲。在 RE 之前、3 组和 6 组深蹲后以及 RE 后 5、15、30 和 70 分钟时采集血液样本。在 RE 之前、之后 10 分钟和 70 分钟时采集股外侧肌的肌肉活检样本。分析血液样本中的总睾酮和游离睾酮浓度，分析肌肉样本中的 AR 和 GR 蛋白含量。循环总睾酮在男性中显著增加（p<或=0.05），游离睾酮在男性和女性中均在运动后增加。与运动前相比，男性在运动后 70 分钟时 AR 显著降低，女性在运动后 10 分钟时 AR 显著降低。RE 后 GR 没有变化，但女性的 GR 明显高于男性。这些发现支持了 AR 对 RE 的急性反应的稳定后减少然后反弹的当前范式，但表明在 AR 反应的时间线上存在性别差异。
