Op den Brouw Marjoleine L, Binda Rekha S, Geijtenbeek Teunis B H, Janssen Harry L A, Woltman Andrea M
Department of Gastroenterology and Hepatology, Erasmus MC, Rotterdam, The Netherlands.
Virology. 2009 Oct 10;393(1):84-90. doi: 10.1016/j.virol.2009.07.015. Epub 2009 Aug 15.
Dendritic cells (DC) play a key role in anti-viral immunity. Direct interactions between DC and hepatitis B virus (HBV) may explain the impaired DC function and the ineffective anti-viral response of chronic HBV patients resulting in HBV persistence. Here, the interaction between HBV surface antigens (HBsAg) and DC and the receptor involved were examined by flow cytometry in blood and liver tissue of HBV patients. The in vitro data showed that the mannose receptor (MR) is involved in HBsAg recognition and uptake by DC. The presence of HBsAg-positive DC was demonstrated sporadically in blood, but frequently in the liver of HBV patients. Interestingly, a positive correlation was found between HBsAg positivity and MR expression level in both liver- and blood-derived DC. These data suggest that in HBV infected patients, MR-mediated interaction between HBsAg and DC and subsequent impairment of DC predominantly occurs at the main site of infection, the liver.
树突状细胞（DC）在抗病毒免疫中起关键作用。DC与乙型肝炎病毒（HBV）之间的直接相互作用可能解释了慢性HBV患者DC功能受损以及抗病毒反应无效导致HBV持续存在的原因。在此，通过流式细胞术检测了HBV患者血液和肝组织中HBV表面抗原（HBsAg）与DC之间的相互作用以及相关受体。体外数据表明，甘露糖受体（MR）参与DC对HBsAg的识别和摄取。在HBV患者的血液中偶尔能检测到HBsAg阳性的DC，但在肝脏中则较为常见。有趣的是，在源自肝脏和血液的DC中，HBsAg阳性与MR表达水平之间存在正相关。这些数据表明，在HBV感染患者中，MR介导的HBsAg与DC之间的相互作用以及随后DC的损伤主要发生在感染的主要部位——肝脏。
