Key Laboratory of Pollution Processes and Environmental Criteria Nankai University, Ministry of Education, College of Environmental Sciences and Engineering, Nankai University, Tianjin 300071, China.
J Hazard Mater. 2009 Nov 15;171(1-3):1096-102. doi: 10.1016/j.jhazmat.2009.06.132. Epub 2009 Jun 30.
In the present study, direct evidence of hydroxyl radical production in livers of Carassius auratus exposed to pentachlorophenol (PCP) was provided using electron paramagnetic resonance (EPR) with spin-trapping. A dose-effect relationship was obtained between hydroxyl radical intensities and PCP exposure. It was observed that hydroxyl radical was significantly induced by 0.001 mg l(-1) (below the criteria for Chinese fishery water quality) of PCP exposure. A strong positive correlation (r=0.9581, p<0.001) was observed between PCP liver concentrations and hydroxyl radical intensities within 7d of PCP exposure, which suggests that hydroxyl radical are mainly produced from PCP itself. However, no correlation was observed between PCP liver concentrations and hydroxyl radical intensities after 7d, and a higher intensity of hydroxyl radical could still be observed when the PCP liver concentrations decreased to a lower level, which suggests that other mechanisms may possibly contribute to hydroxyl radical production after 7d. The glutathione/oxidized glutathione (GSH/GSSG) ratio decreased below that of the control level during the entire period of PCP exposure (0.05 mg l(-1)), which suggested oxidative stress occurred.
在本研究中，使用电子顺磁共振（EPR）自旋捕获技术，为五氯苯酚（PCP）暴露的鲤鱼肝脏中羟基自由基的产生提供了直接证据。观察到羟基自由基的强度与 PCP 暴露之间存在剂量效应关系。结果表明，0.001mg/L（低于中国渔业水质标准）的 PCP 暴露即可显著诱导产生羟基自由基。在 PCP 暴露的 7 天内，PCP 肝浓度与羟基自由基强度之间存在很强的正相关关系（r=0.9581，p<0.001），这表明羟基自由基主要由 PCP 本身产生。然而，在 7 天后，PCP 肝浓度与羟基自由基强度之间没有相关性，并且当 PCP 肝浓度降低到较低水平时，仍可以观察到较高水平的羟基自由基，这表明在 7 天后可能存在其他机制导致羟基自由基的产生。在整个 PCP 暴露期间（0.05mg/L），谷胱甘肽/氧化型谷胱甘肽（GSH/GSSG）的比值低于对照组水平，这表明发生了氧化应激。
