Hartnell A, Moqbel R, Walsh G M, Bradley B, Kay A B
Department of Allergy and Clinical Immunology, National Heart and Lung Institute, London, U.K.
Immunology. 1990 Feb;69(2):264-70.
We have studied the expression of Fc gamma receptors and leucocyte integrins (CD11/CD18 family) on human eosinophils using specific monoclonal antibodies (mAb) and flow cytometric analysis. Peripheral blood eosinophils of normal density and low density were compared with neutrophils and monocytes. Several properties of the human eosinophil were established. These were (i) that the eosinophil expressed Fc gamma RII (CDw32) only (unlike monocytes which bear Fc gamma RI and Fc gamma RII and neutrophils which bear Fc gamma RII and Fc gamma RIII); (ii) that the absence of Fc gamma RIII (CD16) on eosinophils served as a basis for distinguishing eosinophil and neutrophil populations by immunofluorescence; (iii) that the leucocyte adhesion glycoproteins, LFA-1 alpha (CD11a), CR3-alpha (CD11b), p150, 95-alpha (CD11c) and the common beta-chain (CD18), were expressed on the eosinophil as well as the neutrophil; (iv) that CD18 expression was significantly reduced on low-density eosinophils from the hypereosinophilic syndrome. Thus, our findings emphasize the unique phenotype of the human eosinophil in terms of Fc gamma receptor expression, the similarity of the eosinophil and neutrophil with regard to the leucocyte integrins and that eosinophils of low density do not differ greatly from those of normal density in terms of receptor expression.
我们使用特异性单克隆抗体（mAb）和流式细胞术分析，研究了Fcγ受体和白细胞整合素（CD11/CD18家族）在人嗜酸性粒细胞上的表达。将正常密度和低密度的外周血嗜酸性粒细胞与中性粒细胞和单核细胞进行了比较。确定了人嗜酸性粒细胞的几个特性。这些特性包括：（i）嗜酸性粒细胞仅表达FcγRII（CDw32）（与表达FcγRI和FcγRII的单核细胞以及表达FcγRII和FcγRIII的中性粒细胞不同）；（ii）嗜酸性粒细胞上缺乏FcγRIII（CD16）可作为通过免疫荧光区分嗜酸性粒细胞和中性粒细胞群体的基础；（iii）白细胞黏附糖蛋白LFA-1α（CD11a）、CR3-α（CD11b）、p150、95-α（CD11c）和共同的β链（CD18）在嗜酸性粒细胞和中性粒细胞上均有表达；（iv）在高嗜酸性粒细胞综合征患者的低密度嗜酸性粒细胞上，CD18表达显著降低。因此，我们的研究结果强调了人嗜酸性粒细胞在Fcγ受体表达方面的独特表型，嗜酸性粒细胞和中性粒细胞在白细胞整合素方面的相似性，以及低密度嗜酸性粒细胞在受体表达方面与正常密度嗜酸性粒细胞没有太大差异。
