通过G4 DNA诱导诱变分离缺失等位基因。
Isolation of deletion alleles by G4 DNA-induced mutagenesis.
作者信息
Pontier Daphne B, Kruisselbrink Evelien, Guryev Victor, Tijsterman Marcel
机构信息
Hubrecht Institute-Koninklijke Nederlandse Akademie van Wetenschappen, University Medical Centre Utrecht, Utrecht, The Netherlands.
出版信息
Nat Methods. 2009 Sep;6(9):655-7. doi: 10.1038/nmeth.1362. Epub 2009 Aug 16.
Metazoan genomes contain thousands of sequence tracts that match the guanine-quadruplex (G4) DNA signature G(3)N(x)G(3)N(x)G(3)N(x)G(3), a motif that is intrinsically mutagenic, probably because it can form secondary structures during DNA replication. Here we show how and to what extent this feature can be used to generate deletion alleles of many Caenorhabditis elegans genes.
后生动物基因组包含数千个与鸟嘌呤四链体（G4）DNA 特征序列 G(3)N(x)G(3)N(x)G(3)N(x)G(3) 相匹配的序列片段，该基序具有内在诱变特性，可能是因为它在 DNA 复制过程中能够形成二级结构。在此，我们展示了如何以及在何种程度上可以利用这一特性来生成许多秀丽隐杆线虫基因的缺失等位基因。
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