从基因到功能：秀丽隐杆线虫的遗传工具箱
From genes to function: the C. elegans genetic toolbox.
作者信息
Boulin Thomas, Hobert Oliver
机构信息
Department of Biology, Institut de Biologie de l'École Normale Supérieure, Paris, France.
出版信息
Wiley Interdiscip Rev Dev Biol. 2012 Jan-Feb;1(1):114-37. doi: 10.1002/wdev.1. Epub 2011 Nov 28.
Abstract
This review aims to provide an overview of the technologies which make the nematode Caenorhabditis elegans an attractive genetic model system. We describe transgenesis techniques and forward and reverse genetic approaches to isolate mutants and clone genes. In addition, we discuss the new possibilities offered by genome engineering strategies and next-generation genome analysis tools.
摘要
本综述旨在概述使线虫秀丽隐杆线虫成为有吸引力的遗传模型系统的技术。我们描述了转基因技术以及用于分离突变体和克隆基因的正向和反向遗传方法。此外，我们还讨论了基因组工程策略和新一代基因组分析工具带来的新可能性。
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