采用琼脂稀释法和肉汤微量稀释法比较五种抗真菌药物对皮肤癣菌的体外活性。
Comparison of in vitro activity of five antifungal agents against dermatophytes, using the agar dilution and broth microdilution methods.
作者信息
Mota Crystiane Rodrigues Araújo, Miranda Karla Carvalho, Lemos Janine de Aquino, Costa Carolina Rodrigues, Hasimoto e Souza Lúcia Kioko, Passos Xisto Sena, Meneses e Silva Hildene, Silva Maria do Rosário Rodrigues
机构信息
Tropical Pathology and Public Health Institute, Federal University of Goiás, Goiânia, GO, Brazil.
出版信息
Rev Soc Bras Med Trop. 2009 May-Jun;42(3):250-4. doi: 10.1590/s0037-86822009000300003.
The purpose of this study was to compare the agar dilution and broth microdilution methods for determining the minimum inhibitory concentration (MIC) of fluconazole, itraconazole, ketoconazole, griseofulvin and terbinafine for 60 dermatophyte samples belonging to the species Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum canis. The percentage agreement between the two methods, for all the isolates with < 2 dilutions that were tested was 91.6% for ketoconazole and griseofulvin, 88.3% for itraconazole, 81.6% for terbinafine and 73.3% for fluconazole. One hundred percent agreement was obtained for Trichophyton mentagrophytes isolates evaluated with ketoconazole and griseofulvin. Thus, until a reference method for testing the in vitro susceptibility of dermatophytes is standardized, the similarity of the results between the two methods means that the agar dilution method may be useful for susceptibility testing on these filamentous fungi.
本研究的目的是比较琼脂稀释法和肉汤微量稀释法，以确定氟康唑、伊曲康唑、酮康唑、灰黄霉素和特比萘芬对60株属于红色毛癣菌、须癣毛癣菌和犬小孢子菌的皮肤癣菌样本的最低抑菌浓度（MIC）。对于所有测试的稀释度小于2的分离株，两种方法之间的一致率为：酮康唑和灰黄霉素为91.6%，伊曲康唑为88.3%，特比萘芬为81.6%，氟康唑为73.3%。在用酮康唑和灰黄霉素评估的须癣毛癣菌分离株中，获得了100%的一致率。因此，在皮肤癣菌体外药敏试验的参考方法标准化之前，两种方法结果的相似性意味着琼脂稀释法可能有助于对这些丝状真菌进行药敏试验。
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