Laboratory of Medical Genetics, Harbin Medical University, 150081 Harbin, China.
Mol Biol Rep. 2010 Jun;37(5):2347-54. doi: 10.1007/s11033-009-9740-y. Epub 2009 Aug 15.
Interphase chromosomes form distinct spatial domains called chromosome territories (CTs). The arrangement of CTs is non-random and correlated with cellular processes such as differentiation. The purpose of this study is to provide some behavior information of CTs during lymphocyte EBV-infection, which is thought to be a general extra-biological model. Three-dimensional fluorescence in situ hybridization (3D-FISH) was performed on human lymphocytes every 24 h over 96 h periods in EBV-infection. Chromosomes 17 and 18 were selected as target territories for similar size and different gene density. The data indicate that the radial position of territories 17 was altered with time, whereas territories 18 showed relative stable localization. The relative CT volume of CTs 18 to 17 also changed with infection. Our study is the first to examine the timely changes of chromatin positioning and folding in EBV-lymphocyte infection. Dynamic changes in position and folding status of target chromosomes reflected an impact of EBV infection on genome stability.
间期染色体形成独特的空间域，称为染色体区域（CTs）。CT 的排列不是随机的，与细胞分化等过程相关。本研究的目的是提供淋巴细胞 EBV 感染过程中 CT 行为的一些信息，这被认为是一种普遍的非生物模型。对 EBV 感染 96 小时内每 24 小时的人类淋巴细胞进行三维荧光原位杂交（3D-FISH）。选择染色体 17 和 18 作为目标区域，因为它们大小相似但基因密度不同。数据表明，区域 17 的径向位置随时间发生改变，而区域 18 表现出相对稳定的定位。CTs 18 相对于 CTs 17 的相对 CT 体积也随感染而变化。本研究首次检测了 EBV-淋巴细胞感染中染色质定位和折叠的时变。靶染色体位置和折叠状态的动态变化反映了 EBV 感染对基因组稳定性的影响。
