Asakawa Haruhiko, Hiraoka Yasushi
Graduate School of Frontier Biosciences, Osaka University, Osaka, Suita, Japan.
Methods Mol Biol. 2009;558:53-64. doi: 10.1007/978-1-60761-103-5_4.
The fission yeast Schizosaccharomyces pombe has provided a useful experimental system to study nuclear structures during meiosis. Unlike many higher animals in which meiosis takes place only in specialized tissues deep inside their bodies, S. pombe is a unicellular eukaryote and its meiosis can be induced simply by depleting nitrogen sources from the culture medium. The entire process of meiosis is completed within several hours, and thus can be followed in individual living cells. These features provide ease of microscopic observation. A more trivial merit is its rod-like cell shape, which aids microscopic observation, as the long axis of cells is kept in the microscope image plane. Here we describe methods for induction of meiosis and fluorescence microscopy observation in living cells of S. pombe.
裂殖酵母粟酒裂殖酵母为研究减数分裂过程中的核结构提供了一个有用的实验系统。与许多高等动物不同，高等动物的减数分裂仅在其体内深处的特化组织中进行，而粟酒裂殖酵母是一种单细胞真核生物，其减数分裂可通过从培养基中耗尽氮源来简单诱导。减数分裂的整个过程在数小时内完成，因此可以在单个活细胞中进行跟踪观察。这些特性便于显微镜观察。一个更不起眼的优点是它的杆状细胞形状，这有助于显微镜观察，因为细胞的长轴保持在显微镜图像平面内。在这里，我们描述了粟酒裂殖酵母活细胞中减数分裂诱导和荧光显微镜观察的方法。
