Peckham C S, Tedder R S, Briggs M, Ades A E, Hjelm M, Wilcox A H, Parra-Mejia N, O'Connor C
Department of Paediatric Epidemiology, Institute of Child Health, London, UK.
Lancet. 1990 Mar 3;335(8688):516-9. doi: 10.1016/0140-6736(90)90746-r.
This pilot study established that unlinked anonymous testing of dried blood spots routinely collected on Guthrie cards for neonatal screening is a feasible method for monitoring HIV prevalence in women at the time of delivery. The method was sensitive, specific, and less expensive than more conventional ELISAs. 114,515 dried blood spots taken from cards collected in three Thames regions were tested for antibody to HIV-1. 28 samples were confirmed to be antibody positive by western blot (seroprevalence 0.24 per 1000). Unlinked anonymous screening of newborn babies should be extended to monitor the spread of HIV infection in the heterosexual population and to target preventive strategies and provision of health care.
这项试点研究证实，对在用于新生儿筛查的滤纸片（Guthrie卡片）上常规采集的干血斑进行非关联匿名检测，是监测分娩时女性艾滋病毒感染率的一种可行方法。该方法灵敏、特异，且比更传统的酶联免疫吸附测定（ELISA）成本更低。对取自泰晤士河三个地区所收集卡片上的114,515份干血斑进行了HIV-1抗体检测。通过蛋白质印迹法确认28份样本抗体呈阳性（血清阳性率为每1000例中有0.24例）。应扩大对新生儿的非关联匿名筛查，以监测艾滋病毒感染在异性恋人群中的传播情况，并针对预防策略和医疗保健提供目标。
