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百日咳博德特氏菌内毒素“类脂X”组分中2-甲基-3-羟基癸酸和2-甲基-3-羟基十四烷酸的鉴定

Identification of 2-methyl-3-hydroxydecanoic and 2-methyl-3-hydroxytetradecanoic acids in the 'lipid X' fraction of the Bordetella pertussis endotoxin.

作者信息

Haeffner N, Chaby R, Szabó L

出版信息

Eur J Biochem. 1977 Aug 1;77(3):535-44. doi: 10.1111/j.1432-1033.1977.tb11696.x.

DOI:10.1111/j.1432-1033.1977.tb11696.x
PMID:196857
Abstract

The 'lipid X' fraction, released from the Bordetella pertussis endotoxin upon treatment with trifluoroacetic acid of pH 3 at 50 degrees C, was shown to contain, in addition to 3-hydroxydecanoic, 3-hydroxydodecanoic, 3-hydroxytetradecanoic, and tetradec-2-enoic acids, 2-methyl-3-hydroxydecanoic-, and 2-methyl-3-hydroxytetradecanoic acids. The structure of these was established by gas-liquid chromatography/mass spectrometry.

摘要

在50℃下用pH 3的三氟乙酸处理百日咳博德特氏菌内毒素后释放出的“脂质X”部分,除含有3-羟基癸酸、3-羟基十二烷酸、3-羟基十四烷酸和十四碳-2-烯酸外,还含有2-甲基-3-羟基癸酸和2-甲基-3-羟基十四烷酸。通过气-液色谱/质谱法确定了它们的结构。

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