脆弱拟杆菌NCTC 9343及相关菌株脂多糖中(3-羟基)脂肪酸的性质、连接类型、数量和绝对构型
Nature, type of linkage, quantity, and absolute configuration of (3-hydroxy) fatty acids in lipopolysaccharides from Bacteroides fragilis NCTC 9343 and related strains.
作者信息
Wollenweber H W, Rietschel E T, Hofstad T, Weintraub A, Lindberg A A
出版信息
J Bacteriol. 1980 Dec;144(3):898-903. doi: 10.1128/jb.144.3.898-903.1980.
Abstract
The main fatty acids present in lipopolysaccharides from Bacteroides fragilis NCTC 9343 were identified as 13-methyl-tetradecanoic, D-3-hydroxypentadecanoic, D-3-hydroxyhexadecanoic, D-3-hydroxy-15-methyl-hexadecanoic, and D-3-hydroxyheptadecanoic acids. Of these, 13-methyl-tetradecanoic acid is exclusively ester bound, and 3-hydroxy-15-methyl-hexadecanoic acid is exclusively involved in amide linkage. The other 3-hydroxy fatty acids are both ester and amide bound. All 3-hydroxy fatty acids possess the D configuration, and the 3-hydroxyl group of ester-linked 3-hydroxy fatty acids is not substituted. Lipopolysaccharides of related Bacteroides species (B. thetaiotaomicron, B. ovatus, B. distasonis, and B. vulgatus) showed a fatty acid spectrum with both similar and distinct features compared to that of B. fragilis lipopolysaccharides.
摘要
脆弱拟杆菌NCTC 9343脂多糖中存在的主要脂肪酸被鉴定为13-甲基十四烷酸、D-3-羟基十五烷酸、D-3-羟基十六烷酸、D-3-羟基-15-甲基十六烷酸和D-3-羟基十七烷酸。其中，13-甲基十四烷酸仅以酯键结合，3-羟基-15-甲基十六烷酸仅参与酰胺键合。其他3-羟基脂肪酸同时以酯键和酰胺键结合。所有3-羟基脂肪酸均具有D构型，酯键连接的3-羟基脂肪酸的3-羟基未被取代。与脆弱拟杆菌脂多糖相比，相关拟杆菌属物种（多形拟杆菌、卵形拟杆菌、解脲拟杆菌和普通拟杆菌）的脂多糖显示出具有相似和不同特征的脂肪酸谱。
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