[反相高效液相色谱法同时测定白花前胡根中三种香豆素成分]
[Simultaneous determination of three coumarin constituents in roots of Peucedanum praeruptorum by RP-HPLC].
作者信息
Wu Wenling, Chen Jiajia, Liu Shoujin, Yu Nianjun, Fang Chengwu, Zhang Qingqing
机构信息
Anhui Key Laboratory of Modernized Chinese Material, Anhui University of Traditional Chinese Medicine, Hefei 230031, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2009 May;34(9):1121-3.
OBJECTIVE
To develop a reserved-phase HPLC method for the determination of praeruptorin A, praeruptorin B, qianhucoumarin E in roots of Peucedanum praeruptorum.
METHOD
Agilent TC-C18 column (4.6 mm x 250 mm, 5 microm) was used at 30 degrees C with the mobile phase of methanol-water (75:25). The flow rate was set at 0.8 mL x min(-1). The detection wavelength was 321 nm.
RESULT
The linear response ranged from 3.20-28.80 microg for +/- praeruptorin A (r = 0.9999, n = 5), 1.60-14.40 g for praeruptorin B (r = 0.9995, n = 5) and 1.64-14.76 g for qianhucoumarin E (r = 0.9994, n = 5), respectively. Recoveries were 98.92% with RSD 1.6% for praeruptorin A, 99.66% with RSD 1.5% for praeruptorin B and 99.72% with RSD 1.4% for qianhucoumarin E.
CONCLUSION
The method is quick, simple and repeatable for determination of three coumarin constituents in root of P. praeruptorum.
目的
建立一种反相高效液相色谱法，用于测定前胡根中的白花前胡甲素、白花前胡乙素和前胡香豆素E。
方法
采用安捷伦TC-C18柱（4.6 mm×250 mm，5μm），柱温30℃，流动相为甲醇-水（75:25），流速设定为0.8 mL·min⁻¹，检测波长为321 nm。
结果
白花前胡甲素的线性范围为3.20 - 28.80μg（r = 0.9999，n = 5），白花前胡乙素为1.60 - 14.40μg（r = 0.9995，n = 5），前胡香豆素E为1.64 - 14.76μg（r = 0.9994，n = 5）。白花前胡甲素的回收率为98.92%，相对标准偏差为1.6%；白花前胡乙素的回收率为99.66%，相对标准偏差为1.5%；前胡香豆素E的回收率为99.72%，相对标准偏差为1.4%。
结论
该方法用于测定前胡根中三种香豆素成分快速、简便且可重复。
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