Yu Yu-Shan, Hsu Chin-Lin, Yen Gow-Chin
Department of Food Science and Biotechnology, National Chung Hsing University, Taichung 40227, Taiwan.
J Agric Food Chem. 2009 Sep 9;57(17):7673-80. doi: 10.1021/jf901327g.
Alpinia pricei Hayata is cultivated throughout Asia and is an endemic plant in Taiwan. The leaf and root of this plant are used for traditional wrapping of food and as a cooking substitute for fresh ginger. The aim of this work was to study the in vitro anti-inflammatory effects of ethanol extracts from A. pricei Hayata (EEAP) and its phenolic compounds. High-performance liquid chromatography (HPLC) profiling indicated that EEAP contained caffeic acid, chlorogenic acid, ferulic acid, p-hydroxybenzoic acid, rutin, apigenin, curcumin and pinocembrin. EEAP and its phenolic compounds, apigenin, curcumin, and pinocembrin, inhibited lipopolysaccharide (LPS)-stimulated nitric oxide (NO) and prostaglandin E(2) (PGE(2)) production in RAW 264.7 cells. Furthermore, EEAP, apigenin, curcumin, and pinocembrin decreased LPS-mediated induction of protein and mRNA expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in RAW 264.7 cells. In addition, EEAP and its major active compound pinocembrin inhibited LPS-induced nuclear translocation of nuclear factor-kappaB (NF-kappaB) and NF-kappaB-mediated reporter gene expression. EEAP and pinocembrin also significantly inhibited LPS-induced intracellular reactive oxygen species (ROS) production in RAW 264.7 cells. When these results are taken together, they indicate that EEAP and pinocembrin suppressed LPS-induced NO and PGE(2) production by inhibition of NF-kappaB nuclear translocation and ROS generation.
台东月桃在亚洲各地均有种植，是台湾的一种本土植物。这种植物的叶子和根在传统上用于包裹食物，也可作为新鲜生姜的烹饪替代品。本研究的目的是探讨台东月桃乙醇提取物（EEAP）及其酚类化合物的体外抗炎作用。高效液相色谱（HPLC）分析表明，EEAP含有咖啡酸、绿原酸、阿魏酸、对羟基苯甲酸、芦丁、芹菜素、姜黄素和松属素。EEAP及其酚类化合物芹菜素、姜黄素和松属素可抑制脂多糖（LPS）刺激的RAW 264.7细胞中一氧化氮（NO）和前列腺素E2（PGE2）的产生。此外，EEAP、芹菜素、姜黄素和松属素可降低LPS介导的RAW 264.7细胞中诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2）蛋白和mRNA表达的诱导。此外，EEAP及其主要活性化合物松属素可抑制LPS诱导的核因子-κB（NF-κB）核转位和NF-κB介导的报告基因表达。EEAP和松属素还显著抑制LPS诱导的RAW 264.7细胞内活性氧（ROS）的产生。综合这些结果表明，EEAP和松属素通过抑制NF-κB核转位和ROS生成来抑制LPS诱导的NO和PGE2的产生。
