对感染金黄色葡萄球菌的免疫抑制小鼠预防性给予噬菌体的效果。
Effects of prophylactic administration of bacteriophages to immunosuppressed mice infected with Staphylococcus aureus.
作者信息
Zimecki Michał, Artym Jolanta, Kocieba Maja, Weber-Dabrowska Beata, Borysowski Jan, Górski Andrzej
机构信息
Institute of Immunology and Experimental Therapy, Polish Academy of Science, Wrocław, Poland.
出版信息
BMC Microbiol. 2009 Aug 17;9:169. doi: 10.1186/1471-2180-9-169.
BACKGROUND
Bacteriophages can be successfully applied to treat infections caused by antibiotic-resistant bacteria. Until now no attempts have been undertaken to treat infections in immunosuppressed patients with phages. In this work we investigated the prophylactic efficacy of specific bacteriophages in CBA mice treated with cyclophosphamide (CP) and infected with Staphylococcus aureus.
RESULTS
High numbers of bacterial colony-forming units in the organs as well as elevated tumor necrosis factor and interleukin-6 serum concentrations in CP-treated and S. aureus-infected mice were significantly lowered upon application of phages. The phages markedly increased the percentage of circulating neutrophils and immature cells from the myelocytic and lymphocytic lineages in CP-treated, S. aureus-infected mice as well as of myelocytes and immature neutrophils in the bone marrow. In addition, phages stimulated in such mice generation of specific agglutinins against S. aureus.
CONCLUSION
Application of specific phages to immunosuppressed mice prior to infection with S. aureus proved very effective, suggesting a potential benefit of phage therapy in immunocompromised patients experiencing bacterial infections.
背景
噬菌体可成功应用于治疗由耐抗生素细菌引起的感染。迄今为止，尚未尝试用噬菌体治疗免疫抑制患者的感染。在本研究中，我们调查了特定噬菌体对用环磷酰胺（CP）处理并感染金黄色葡萄球菌的CBA小鼠的预防效果。
结果
在用噬菌体处理后，CP处理且感染金黄色葡萄球菌的小鼠器官中大量的细菌菌落形成单位以及血清中升高的肿瘤坏死因子和白细胞介素-6浓度均显著降低。噬菌体显著增加了CP处理、感染金黄色葡萄球菌小鼠中循环中性粒细胞以及来自髓细胞系和淋巴细胞系的未成熟细胞的百分比，以及骨髓中髓细胞和未成熟中性粒细胞的百分比。此外，噬菌体刺激此类小鼠产生针对金黄色葡萄球菌的特异性凝集素。
结论
在感染金黄色葡萄球菌之前将特定噬菌体应用于免疫抑制小鼠被证明非常有效，这表明噬菌体疗法对经历细菌感染的免疫受损患者可能有益。
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