Drug Delivery Research Laboratory, Department of Pharmaceutical Sciences, Dr. Harisingh Gour Vishwavidyalaya, Sagar, MP 470003, India.
J Control Release. 2009 Dec 3;140(2):157-65. doi: 10.1016/j.jconrel.2009.08.004. Epub 2009 Aug 15.
The aim of present work was to investigate the potential utility of novel carrier gel core liposomes for intramuscular delivery of transmission blocking malaria antigen Pfs25 and to evaluate the effect of co-administration of vaccine adjuvant CpGODN on immune enhancement of recombinant protein antigen Pfs25. In the present work we have prepared gel core liposomes containing core of biocompatible polymer poly acrylic acid in phospholipid bilayer by reverse phase evaporation method and characterized for various in vitro parameters. In process stability of the encapsulated antigen was evaluated by SDS-PAGE followed by western blotting. The immune stimulating ability was studied by measuring anti-Pfs25 antibody titer in serum of Balb/c mice following intramuscular administration of various formulations. A Significant and perdurable immune responses was obtained after intramuscular administration of gel core liposomes encapsulated Pfs25 as compared to Pfs25 loaded conventional liposomes. Moreover co-administration of CpGODN in liposomes (conventional and gel core) was found to further increase the immunogenicity of vaccine. The result indicates high potential of gel core liposomes for their use as a carrier adjuvant for intramuscular delivery of recombinant antigen Pfs25 based transmission blocking malaria vaccine.
本研究旨在探索新型载体制剂凝胶芯脂质体作为肌肉内传递传播阻断性疟疾抗原 Pfs25 的潜在应用，并评估疫苗佐剂 CpGODN 共同给药对重组蛋白抗原 Pfs25 免疫增强的影响。在本工作中，我们通过反相蒸发法制备了含有生物相容性聚合物聚丙烯酸核的凝胶芯脂质体，并对其进行了各种体外参数的表征。通过 SDS-PAGE 结合 Western blot 评估了包封抗原的过程稳定性。通过测量 Balb/c 小鼠肌肉内给予各种制剂后血清中的抗 Pfs25 抗体滴度来研究其免疫刺激能力。与负载 Pfs25 的传统脂质体相比，肌肉内给予凝胶芯脂质体包封的 Pfs25 后可获得显著且持久的免疫应答。此外，CpGODN 在脂质体（传统和凝胶芯）中的共同给药被发现进一步增加了疫苗的免疫原性。结果表明，凝胶芯脂质体具有作为基于肌肉内传递的重组抗原 Pfs25 的传播阻断性疟疾疫苗的载体佐剂的高潜力。
