从大熊猫（Ailuropoda melanoleuca）中分离和鉴定犬冠状病毒毒株。
Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca).
作者信息
Gao Feng-Shan, Hu Gui-Xue, Xia Xian-zhu, Gao Yu-Wei, Bai Ya-Duo, Zou Xiao-Huan
机构信息
Department of Biochemistry and Molecular Biology, College of Bioengineering, Dalian University, Dalian, Liaoning 116622, China.
出版信息
J Vet Sci. 2009 Sep;10(3):261-3. doi: 10.4142/jvs.2009.10.3.261.
Abstract
Two giant pandas (Ailuropoda melanoleuca) died of unknown causes in a Chinese zoo. The clinical disease profile suggested that the pandas may have suffered a viral infection. Therefore, a series of detection including virus isolation, electron microscopy, cytobiological assay, serum neutralization and RT-PCR were used to identify the virus. It was determined that the isolated virus was a canine coronavirus (CCV), on the basis of coronavirus, neutralization by canine anti-CCV serum, and 84.3% to 100% amino acid sequence similarity with CCV. The results suggest that the affected pandas had been infected with CCV.
摘要
在中国一家动物园里，两只大熊猫（大熊猫）不明原因死亡。临床疾病特征表明，这些大熊猫可能感染了病毒。因此，采用了一系列检测方法，包括病毒分离、电子显微镜检查、细胞生物学检测、血清中和试验和逆转录-聚合酶链反应（RT-PCR）来鉴定病毒。根据冠状病毒、犬抗犬冠状病毒血清的中和作用以及与犬冠状病毒84.3%至100%的氨基酸序列相似性，确定分离出的病毒为犬冠状病毒（CCV）。结果表明，受影响的大熊猫感染了犬冠状病毒。
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