Department of Pharmacology, Université de Montréal, Montréal, Quebec, Canada.
Int J Obes (Lond). 2009 Oct;33(10):1166-73. doi: 10.1038/ijo.2009.149. Epub 2009 Aug 18.
Biotransformation of blueberry juice by the Serratia vaccinii bacterium gave rise to adenosine monophosphate-activated protein kinase (AMPK) phosphorylation and glucose uptake in muscle cells and adipocytes, but inhibited adipogenesis. This study investigated the antiobesity and antidiabetic potential of biotransformed blueberry juice (BJ) in KKA(y) mice, rodent model of leptin resistance.
BJ was incorporated in drinking water of KKA(y) mice. Parameters of body weight, food intake, plasma glucose, insulin, leptin, and adiponectin were measured. Before and after therapy, animals were subjected to an oral glucose tolerance test. At the end of treatment, liver, muscle, kidney, epididymal fat pad, abdominal fat pad, and dorsal fat pad were collected and weighed.
Incorporating BJ in drinking water protected young KKA(y) mice from hyperphagia and significantly reduced their weight gain. Moreover, BJ protected young KKA(y) mice against the development of glucose intolerance and diabetes mellitus. Chronic BJ administration in obese and diabetic KKA(y) mice reduced food intake and body weight. This effect could not fully explain the associated antidiabetic effect because BJ-treated mice still showed lower blood glucose level when compared with pair-fed controls. The adipokines pathway also seems to be involved because BJ significantly increased adiponectin levels in obese mice.
This study shows that BJ decreases hyperglycemia in diabetic mice, at least in part by reversing adiponectin levels. BJ also protects young pre-diabetic mice from developing obesity and diabetes. Thus, BJ may represent a novel complementary therapy and a source of novel therapeutic agents against diabetes mellitus.
蓝莓汁经沙雷氏菌转化后，可使肌肉细胞和脂肪细胞中的一磷酸腺苷激活的蛋白激酶（AMPK）磷酸化和葡萄糖摄取增加，同时抑制脂肪生成。本研究旨在探讨经转化的蓝莓汁（BJ）在肥胖型糖尿病 KKA(y) 小鼠（瘦素抵抗的啮齿动物模型）中的抗肥胖和抗糖尿病潜力。
将 BJ 掺入 KKA(y) 小鼠的饮用水中。测量体重、食物摄入量、血浆葡萄糖、胰岛素、瘦素和脂联素等参数。治疗前后，动物均进行口服葡萄糖耐量试验。治疗结束时，采集并称重肝脏、肌肉、肾脏、附睾脂肪垫、腹部脂肪垫和背部脂肪垫。
将 BJ 掺入饮用水中可保护年轻的 KKA(y) 小鼠免受过度摄食，并显著降低其体重增加。此外，BJ 可保护年轻的 KKA(y) 小鼠免受葡萄糖耐量受损和糖尿病的发展。BJ 持续治疗肥胖和糖尿病 KKA(y) 小鼠可减少食物摄入量和体重。由于 BJ 治疗的小鼠与配对喂养的对照组相比，其血糖水平仍较低，因此这种效果不能完全解释其相关的抗糖尿病作用。此外，脂肪细胞因子途径似乎也参与其中，因为 BJ 可显著增加肥胖小鼠的脂联素水平。
本研究表明，BJ 可降低糖尿病小鼠的高血糖水平，至少部分是通过逆转脂联素水平来实现的。BJ 还可保护年轻的糖尿病前期小鼠免于肥胖和糖尿病的发生。因此，BJ 可能代表一种新的补充治疗方法和一种新的抗糖尿病治疗药物的来源。
