Nikaido T, Shimada K, Shibata M, Hata M, Sakamoto M, Takasaki Y, Sato C, Takahashi T, Nishida Y
Molecular Biology Unit, Aichi Cancer Centre Research Institute, Nagoya, Japan.
Clin Exp Immunol. 1990 Feb;79(2):209-14. doi: 10.1111/j.1365-2249.1990.tb05180.x.
Patients with systemic lupus erythematosus (SLE) produce autoantibodies against a variety of nuclear antigens including Ki antigen. Although anti-Ki autoantibodies were found in a significant number of SLE patients, the nature of Ki antigen is poorly characterized. By using anti-Ki serum as a probe we have cloned a bovine cDNA directing the synthesis in Escherichia coli of a polypeptide immunologically indistinguishable from the authentic Ki antigen. A homologous human cDNA was also cloned and its nucleotide sequence predicted the entire primary structure of a novel nuclear protein with a molecular weight of 29 508 and with highly hydrophilic and weakly acidic character. The gene is highly conserved not only in the coding region but also in the 3'-untranslated region. The bacterially produced Ki antigen would be valuable for diagnosis of SLE.
系统性红斑狼疮（SLE）患者会产生针对多种核抗原（包括Ki抗原）的自身抗体。尽管在大量SLE患者中发现了抗Ki自身抗体，但Ki抗原的性质却鲜为人知。我们以抗Ki血清为探针，克隆了一个牛源cDNA，该cDNA可指导大肠杆菌合成一种在免疫学上与天然Ki抗原无法区分的多肽。我们还克隆了一个同源的人源cDNA，其核苷酸序列预测了一种分子量为29508、具有高度亲水性和弱酸性特征的新型核蛋白的完整一级结构。该基因不仅在编码区高度保守，在3'非翻译区也高度保守。细菌产生的Ki抗原对SLE的诊断具有重要价值。
