Fox J C, McNally N J
Department of Urology, Royal Free Hospital School of Medicine, Hampstead, London, UK.
Int J Radiat Biol. 1990 Mar;57(3):513-21. doi: 10.1080/09553009014552651.
The induction and repair of DNA double-strand breaks (DSB) following exposure to 238Pu alpha-particles was examined in V79-379A cells. The technique of neutral filter elution was used in these investigations at both pH 9.6 and pH 7.2. The initial dsb yield was found to be similar to that seen after 250 kVp X-ray or 2.3 MeV neutron exposure. However, the pattern of dsb rejoining after alpha-particle irradiation did not follow that seen after X-rays or neutrons. A very fast initial component, complete within 2 min of incubation following irradiation, removed 70 per cent of the dsb seen after 40 Gy alpha-particles; very little slow rejoining was seen. This contrasts sharply with the dsb rejoining seen after X-ray or neutron exposure, and presumably reflects the differences in the nature of the dsb induced and the way they are repaired.
在V79 - 379A细胞中检测了暴露于238Puα粒子后DNA双链断裂（DSB）的诱导和修复情况。在这些研究中，在pH 9.6和pH 7.2条件下均使用了中性滤膜洗脱技术。发现初始DSB产量与250 kVp X射线或2.3 MeV中子照射后所见相似。然而，α粒子照射后DSB重新连接的模式与X射线或中子照射后不同。一个非常快速的初始成分，在照射后孵育2分钟内完成，去除了40 Gyα粒子后所见DSB的70%；几乎没有观察到缓慢的重新连接。这与X射线或中子照射后所见的DSB重新连接形成鲜明对比，大概反映了诱导的DSB性质及其修复方式的差异。
