Prise K M, Davies S, Michael B D
Cancer Research Campaign, Gray Laboratory, Mount Vernon Hospital, Middlesex, U.K.
Int J Radiat Biol Relat Stud Phys Chem Med. 1987 Dec;52(6):893-902. doi: 10.1080/09553008714552481.
Using the neutral filter elution technique, the induction of DNA double-strand breaks (dsb) has been measured in 250 kVp X-irradiated V79-379A Chinese hamster cells irradiated under air or nitrogen. The dose-effect curves for induced dsb were curvilinear, mirroring cell survival curves, such that there was an approximately linear relationship between induced dsb and lethal lesions (-In (cell survival)) which was independent of oxygen. With cells irradiated with 2.3 MeV neutrons or 238Pu alpha-particles the correlations between lethal events and dsb, although also approximately linear, do not match those for X-rays. With neutrons there is approximately a 2.5-fold reduction in the level of dsb induction per lethal event. Thus either the apparently linear relationships found are spurious, and there is no general correlation between induced dsb and lethal effect, or there are qualitative differences between neutron, alpha-particle and X-ray induced dsb that give them differing probabilities of cell kill.
使用中性滤膜洗脱技术，已测量了在空气或氮气环境下，经250 kVp X射线照射的中国仓鼠V79 - 379A细胞中DNA双链断裂（dsb）的诱导情况。诱导dsb的剂量效应曲线呈曲线状，与细胞存活曲线相似，因此诱导dsb与致死性损伤（-In（细胞存活））之间存在近似线性关系，且该关系与氧气无关。在用2.3 MeV中子或238Puα粒子照射的细胞中，致死事件与dsb之间的相关性虽然也近似线性，但与X射线照射的情况不匹配。对于中子，每个致死事件的dsb诱导水平大约降低2.5倍。因此，要么所发现的明显线性关系是虚假的，诱导dsb与致死效应之间不存在普遍相关性，要么中子、α粒子和X射线诱导的dsb存在质的差异，导致它们产生细胞杀伤的概率不同。
