Kawabata Akiko, Tang Huamin, Huang Honglan, Yamanishi Koichi, Mori Yasuko
Division of Biomedical Research, National Institute of Biomedical Innovation, Ibaraki, Osaka, Japan.
Virol J. 2009 Aug 19;6:127. doi: 10.1186/1743-422X-6-127.
In general, enveloped viruses are highly dependent on their lipid envelope for entry into host cells. Here, we demonstrated that during the course of virus maturation, a significant proportion of human herpesvirus 6 (HHV-6) envelope proteins were selectively concentrated in the detergent-resistant glycosphingolipid- and cholesterol-rich membranes (rafts) in HHV-6-infected cells. In addition, the ganglioside GM1, which is known to partition preferentially into lipid rafts, was detected in purified virions, along with viral envelope glycoproteins, gH, gL, gB, gQ1, gQ2 and gO indicating that at least one raft component was included in the viral particle during the assembly process.
一般来说，包膜病毒高度依赖其脂质包膜进入宿主细胞。在此，我们证明，在病毒成熟过程中，相当一部分人疱疹病毒6型（HHV - 6）包膜蛋白选择性地集中在HHV - 6感染细胞中耐去污剂的富含糖鞘脂和胆固醇的膜（脂筏）中。此外，在纯化的病毒粒子中检测到神经节苷脂GM1，其已知优先分配到脂筏中，同时还有病毒包膜糖蛋白gH、gL、gB、gQ1、gQ2和gO，这表明在组装过程中病毒粒子包含至少一种脂筏成分。
