Huang Honglan, Li Yongmei, Sadaoka Tomohiko, Tang Huanmin, Yamamoto Takahito, Yamanishi Koichi, Mori Yasuko
Department of Microbiology, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation, 7-6-8 Saito-Asagi, Ibaraki, Osaka 567-0085, Japan.
J Gen Virol. 2006 Feb;87(Pt 2):277-285. doi: 10.1099/vir.0.81551-0.
In this study, the role of cholesterol in the envelope of human herpesvirus 6 (HHV-6) was examined by using methyl-beta-cyclodextrin (MbetaCD) depletion. When cholesterol was removed from HHV-6 virions with MbetaCD, infectivity was abolished, but it could be rescued by the addition of exogenous cholesterol. HHV-6 binding was affected slightly by MbetaCD treatment. In contrast, envelope cholesterol depletion markedly affected HHV-6 infectivity and HHV-6-induced cell fusion. These results suggest that the cholesterol present in the HHV-6 envelope plays a prominent role in the fusion process and is a key component in viral entry.
在本研究中，通过使用甲基-β-环糊精（MβCD）去除胆固醇，研究了胆固醇在人疱疹病毒6型（HHV-6）包膜中的作用。当用MβCD从HHV-6病毒粒子中去除胆固醇时，感染力消失，但可通过添加外源性胆固醇来挽救。MβCD处理对HHV-6结合有轻微影响。相比之下，包膜胆固醇的去除显著影响HHV-6的感染力和HHV-6诱导的细胞融合。这些结果表明，HHV-6包膜中的胆固醇在融合过程中起重要作用，是病毒进入的关键成分。
