人疱疹病毒6型的包膜胆固醇是病毒进入所必需的。

Human herpesvirus 6 envelope cholesterol is required for virus entry.

作者信息

Huang Honglan, Li Yongmei, Sadaoka Tomohiko, Tang Huanmin, Yamamoto Takahito, Yamanishi Koichi, Mori Yasuko

机构信息

Department of Microbiology, Osaka University, Graduate School of Medicine, Suita, Osaka 565-0871, Japan.

Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation, 7-6-8 Saito-Asagi, Ibaraki, Osaka 567-0085, Japan.

出版信息

J Gen Virol. 2006 Feb;87(Pt 2):277-285. doi: 10.1099/vir.0.81551-0.

DOI:10.1099/vir.0.81551-0

PMID:16432012

Abstract

In this study, the role of cholesterol in the envelope of human herpesvirus 6 (HHV-6) was examined by using methyl-beta-cyclodextrin (MbetaCD) depletion. When cholesterol was removed from HHV-6 virions with MbetaCD, infectivity was abolished, but it could be rescued by the addition of exogenous cholesterol. HHV-6 binding was affected slightly by MbetaCD treatment. In contrast, envelope cholesterol depletion markedly affected HHV-6 infectivity and HHV-6-induced cell fusion. These results suggest that the cholesterol present in the HHV-6 envelope plays a prominent role in the fusion process and is a key component in viral entry.

摘要

在本研究中，通过使用甲基-β-环糊精（MβCD）去除胆固醇，研究了胆固醇在人疱疹病毒6型（HHV-6）包膜中的作用。当用MβCD从HHV-6病毒粒子中去除胆固醇时，感染力消失，但可通过添加外源性胆固醇来挽救。MβCD处理对HHV-6结合有轻微影响。相比之下，包膜胆固醇的去除显著影响HHV-6的感染力和HHV-6诱导的细胞融合。这些结果表明，HHV-6包膜中的胆固醇在融合过程中起重要作用，是病毒进入的关键成分。