Pletnev Sergei, Morozova Kateryna S, Verkhusha Vladislav V, Dauter Zbigniew
SAIC-Frederick Inc., Basic Research Program, Argonne National Laboratory, Argonne, IL 60439, USA.
Acta Crystallogr D Biol Crystallogr. 2009 Sep;65(Pt 9):906-12. doi: 10.1107/S0907444909020927. Epub 2009 Aug 6.
In the last decade, advances in instrumentation and software development have made crystallography a powerful tool in structural biology. Using this method, structural information can now be acquired from pathological crystals that would have been abandoned in earlier times. In this paper, the order-disorder (OD) structure of fluorescent protein FP480 is discussed. The structure is composed of tetramers with 222 symmetry incorporated into the lattice in two different ways, namely rotated 90 degrees with respect to each other around the crystal c axis, with tetramer axes coincident with crystallographic twofold axes. The random distribution of alternatively oriented tetramers in the crystal creates a rotational OD structure with statistically averaged I422 symmetry, although the presence of very weak and diffuse additional reflections suggests that the randomness is only approximate.
在过去十年中，仪器设备和软件开发方面的进展使晶体学成为结构生物学中的一种强大工具。使用这种方法，现在可以从过去会被舍弃的病理性晶体中获取结构信息。本文讨论了荧光蛋白FP480的有序-无序（OD）结构。该结构由具有222对称性的四聚体组成，以两种不同方式纳入晶格，即围绕晶体c轴彼此旋转90度，四聚体轴与晶体学二重轴重合。晶体中交替取向的四聚体的随机分布产生了具有统计平均I422对称性的旋转OD结构，尽管非常微弱和弥散的额外反射的存在表明这种随机性只是近似的。
