Department of Nephrology, Medical School, University of Ioannina, Ioannina, Greece.
Nephron Clin Pract. 2009;113(3):c234-40. doi: 10.1159/000235244. Epub 2009 Aug 18.
BACKGROUND/AIMS: Oxidative damage has been reported to be involved in the pathophysiology of chronic kidney disease (CKD) as well as in the pathogenesis of cardiovascular complications of CKD patients. The aim of the present investigation was to evaluate the levels of plasma carbonyl formation, a sensitive marker of enhanced oxidative stress in predialysis, hemodialysis (HD) and peritoneal dialysis (PD) patients.
Plasma samples from 20 apparently healthy control individuals and 127 CKD (stages 2, 3, 4, HD and PD) patients were evaluated by Western blot analysis for the estimation of the levels of protein carbonyl formation.
Albumin represented the main plasma carbonylated protein. Increasing carbonylation of albumin was detected along with the severity of CKD, reaching significance at stages 3 and 4 (p < 0.01, compared to healthy controls). The carbonylation of albumin was even higher in the plasma of HD patients (p < 0.001), while in PD patients it was not statistically significant compared to controls (p = 0.224).
The data presented in this work indicate that oxidative stress in CKD patients gradually increased during the development of the disease. This stress is probably intensified during HD, but not in PD subjects.
背景/目的：氧化损伤已被报道与慢性肾脏病（CKD）的病理生理学以及 CKD 患者心血管并发症的发病机制有关。本研究旨在评估血浆羰基形成水平，这是一种评估增强的氧化应激的敏感标志物在透析前、血液透析（HD）和腹膜透析（PD）患者中的作用。
通过 Western blot 分析评估来自 20 名健康对照个体和 127 名 CKD（2 期、3 期、4 期、HD 和 PD）患者的血浆样本，以评估蛋白羰基形成水平。
白蛋白是主要的血浆羰基化蛋白。随着 CKD 的严重程度的增加，白蛋白的羰基化程度也随之增加，在 3 期和 4 期时达到显著水平（与健康对照组相比，p < 0.01）。HD 患者的白蛋白羰基化程度更高（p < 0.001），而与对照组相比，PD 患者的羰基化程度没有统计学意义（p = 0.224）。
本工作中提出的数据表明，CKD 患者的氧化应激在疾病发展过程中逐渐增加。这种应激在 HD 期间可能会加剧，但在 PD 患者中则不会。
