Department of Wood Science, University of British Columbia, 4030-2424 Main Mall, Vancouver, BC V6T 1Z4, Canada.
Transgenic Res. 2010 Apr;19(2):269-83. doi: 10.1007/s11248-009-9309-5. Epub 2009 Aug 19.
Nicotiana tabacum (tobacco) was transformed with three genes involved in sucrose metabolism, UDP-glucose pyrophosphorylase (UGPase, EC 2.7.7.9), sucrose synthase (SuSy, EC 2.4.1.13) and sucrose phosphate synthase (SPS, EC 2.4.1.14). Plants harbouring the single transgenes were subsequently crossed to produce double and triple transgenic lines, including: 2 x 35S::UGPase x SPS, 4CL::UGPase x SPS, 2 x 35S::SuSy x SPS, 4CL::SuSy x SPS, 2 x 35S::UGPase x SuSy x SPS, and 4CL::UGPase x SuSy x SPS. The ultimate aim of the study was to examine whether it is possible to alter cellulose production through the manipulation of sucrose metabolism genes. While altering sucrose metabolism using UGPase, SuSy and SPS does not have an end effect on cellulose production, their simultaneous overexpression resulted in enhanced primary growth as seen in an increase in height growth, in some cases over 50%. Furthermore, the pyramiding strategy of simultaneously altering the expression of multiple genes in combination resulted in increased time to reproductive bud formation as well as altered flower morphology and foliar stipule formation in 4CL lines. Upregulation of these sucrose metabolism genes appears to directly impact primary growth and therefore biomass production in tobacco.
烟草（Nicotiana tabacum）被转入了三个参与蔗糖代谢的基因，分别是 UDP-葡萄糖焦磷酸化酶（UGPase，EC 2.7.7.9）、蔗糖合酶（SuSy，EC 2.4.1.13）和蔗糖磷酸合酶（SPS，EC 2.4.1.14）。随后，含有单个转基因的植物被杂交以产生双和三转基因系，包括：2 x 35S::UGPase x SPS、4CL::UGPase x SPS、2 x 35S::SuSy x SPS、4CL::SuSy x SPS、2 x 35S::UGPase x SuSy x SPS 和 4CL::UGPase x SuSy x SPS。该研究的最终目的是检验通过操纵蔗糖代谢基因是否有可能改变纤维素的产生。虽然使用 UGPase、SuSy 和 SPS 改变蔗糖代谢不会对纤维素的产生产生最终影响，但它们的同时过表达导致了主要生长的增强，表现在高度生长的增加，在某些情况下超过 50%。此外，同时改变多个基因表达的基因叠加策略导致了生殖芽形成时间的延长，以及 4CL 系中花形态和叶状托叶形成的改变。这些蔗糖代谢基因的上调似乎直接影响烟草的主要生长，从而影响生物量的产生。
