Department of Respiratory Medicine, Fujian Provincial Hospital, 350001, Fuzhou, Fujian, People's Republic of China.
Mol Biol Rep. 2010 Jun;37(5):2241-7. doi: 10.1007/s11033-009-9711-3. Epub 2009 Aug 19.
Livin, a novel member of inhibitors of apoptosis protein, is highly expressed in tumor tissues. It is a potential target in tumor therapy. Silencing its gene expression has been found to promote tumor cell apoptosis or increase tumor sensitivity to therapies. This paper studied the effect of livin anti-apoptotic activity and examined its molecular mechanisms. In the study, higher levels of cell apoptosis were measured by FACS in the experiment group with livin expression silenced than that in controls (P < 0.05). After livin gene expression was knocked down, cleaved caspase-3 protein was up-regulated but caspase-3 mRNA expression was almost the same, the phosphorylated JNK1 protein was down-regulated but JNK1 mRNA and total JNK1 protein expression was approximately the same too. The results suggest that livin may exert anti-apoptotic action on SPC-A1 by activating JNK1 signaling pathway and inhibiting caspase-3 activation.
生存素（Livin）是凋亡抑制蛋白家族的新成员，在肿瘤组织中高表达，是肿瘤治疗的潜在靶点。沉默其基因表达可促进肿瘤细胞凋亡或增加肿瘤对治疗的敏感性。本文研究了生存素抗凋亡活性及其分子机制。在实验中，通过 FACS 检测到沉默生存素表达的实验组细胞凋亡水平高于对照组（P<0.05）。敲低生存素基因表达后，cleaved caspase-3 蛋白上调，但 caspase-3 mRNA 表达几乎相同，磷酸化 JNK1 蛋白下调，但 JNK1 mRNA 和总 JNK1 蛋白表达几乎相同。结果表明，生存素可能通过激活 JNK1 信号通路和抑制 caspase-3 激活对 SPC-A1 发挥抗凋亡作用。
