Gu Junfei, Ren Lixin, Wang Xiaolu, Qu Changbao, Zhang Yong
Department of Urology, The Second Hospital of Hebei Medical University Shijiazhuang 050000, Hebei, China.
Int J Clin Exp Pathol. 2015 Nov 1;8(11):14034-9. eCollection 2015.
To explore the expressions level of Livin, Survivin and Caspase-3 in prostatic cancer and the relationship among the 3 proteins and the clinicopathological features as well as the correlation among them. Totally, 43 paraffin-embedded prostate cancer tissues obtained from patients who were performed with rectal prostate biopsy or excision and 17 paraffin-embedded prostatic hyperplasia tissues were collected. All the specimens were confirmed by pathology. Immunohistochemistry SP method was used to detect the expressions of Livin, Survivin and Caspase-3 in prostatic cancer compared to hyperplasia tissues. The positive expression rates of both Livin and Survivin in prostatic cancer tissue were higher than those in prostatic hyperplasia tissue (93.02% vs. 64.70%, P < 0.05; 83.72% vs. 35.29%, P < 0.01). However, the positive expression rate of Caspase-3 in prostatic cancer tissue was obviously lower than that in prostatic hyperplasia tissue (25.58% vs. 58.82%, P < 0.01). Both Livin and Survivin expressions in prostatic cancer tissue were related to pathological grading (Gleason scores) (X(2) = 14.000, P = 0.001), but not related to preoperative PSA, clinical stages and distant metastasis (P > 0.05). Capsase-3 expression in prostatic cancer tissue was related to pathological grading (Gleason scores) (X (2) = 14.000, P = 0.001) and clinical stages (X (2) = 4.896, P = 0.027), but not related to preoperative PSA and distant metastasis (P > 0.05). In prostatic cancer tissue, Livin expression had no correlation with Survivin expression (r = 0.127, P = 0.419 > 0.05), but negatively correlated with Caspase-3 expression (r = -0.497, P = 0.001). Survivin expression was negatively correlated with Caspase-3 expression (r = -0.354, P = 0.020). Livin, Survivin and Caspase-3 are closely related to the occurrence and development of prostatic cancer and which are expected to become new targets for diagnosis and treatment in future.
探讨Livin、Survivin及Caspase-3在前列腺癌中的表达水平，以及这3种蛋白之间的关系与临床病理特征的关系及其相互之间的相关性。共收集43例经直肠前列腺穿刺活检或切除患者的石蜡包埋前列腺癌组织及17例石蜡包埋前列腺增生组织。所有标本均经病理证实。采用免疫组织化学SP法检测前列腺癌组织与增生组织中Livin、Survivin及Caspase-3的表达。前列腺癌组织中Livin和Survivin的阳性表达率均高于前列腺增生组织（93.02% 对64.70%，P < 0.05；83.72% 对35.29%，P < 0.01）。然而，前列腺癌组织中Caspase-3的阳性表达率明显低于前列腺增生组织（25.58% 对58.82%，P < 0.01）。前列腺癌组织中Livin和Survivin的表达均与病理分级（Gleason评分）有关（X(2)=14.000，P = 0.001），但与术前PSA、临床分期及远处转移无关（P > 0.05）。前列腺癌组织中Caspase-3的表达与病理分级（Gleason评分）有关（X(2)=14.000，P = 0.001）及临床分期（X(2)=4.896，P = 0.027），但与术前PSA及远处转移无关（P > 0.05）。在前列腺癌组织中，Livin的表达与Survivin的表达无相关性（r = 0.127，P = 0.419 > 0.05），但与Caspase-3的表达呈负相关（r = -0.497，P = 0.001）。Survivin的表达与Caspase-3的表达呈负相关（r = -0.354，P = 0.020）。Livin、Survivin及Caspase-3与前列腺癌的发生发展密切相关，有望成为未来诊断和治疗的新靶点。
