Health and Nutrition, Danisco Finland, Kantvik, Finland.
Benef Microbes. 2010 Mar;1(1):81-91. doi: 10.3920/BM2009.0025.
A semi-continuous, anaerobic colon simulator, with four vessels mimicking the conditions of the human large intestine, was used to study the fermentation of xylo-oligosaccharides (XOS). Three XOS compounds and a xylan preparation were fermented for 48 hours by human colonic microbes. Fructo-oligosaccharides (FOS) were used as a prebiotic reference. As a result of the fermentation, the numbers of Bifidobacterium increased in all XOS and xylan simulations when compared to the growth observed in the baseline simulations, and increased levels of Bifidobacterium lactis were measured with the two XOS compounds that had larger distribution of the degree of polymerisation. Fermentation of XOS and xylan increased the microbial production of short chain fatty acids in the simulator vessels; especially the amounts of butyrate and acetate were increased. XOS was more efficient than FOS in increasing the numbers of B. lactis in the colonic model, whereas FOS increased the Bifidobacterium longum numbers more. The selective fermentation of XOS by B. lactis has been demonstrated in pure culture studies, and these results further indicate that the combination of B. lactis and XOS would form a successful, selective synbiotic combination.
采用四个模拟人体大肠条件的容器的半连续厌氧结肠模拟器，研究了木低聚糖（XOS）的发酵情况。将三种 XOS 化合物和木聚糖制剂通过人体结肠微生物发酵 48 小时。低聚果糖（FOS）被用作参考的益生元。结果显示，与基线模拟观察到的生长相比，在所有 XOS 和木聚糖模拟中，双歧杆菌的数量都增加了，而且在两种聚合度分布较大的 XOS 化合物中，测量到了更高水平的双歧杆菌 lactis。XOS 和木聚糖的发酵增加了模拟器容器中微生物产生的短链脂肪酸；特别是丁酸盐和乙酸盐的含量增加了。XOS 比 FOS 更有效地增加了结肠模型中 B. lactis 的数量，而 FOS 则使双歧杆菌长的数量增加更多。在纯培养研究中已经证明了 B. lactis 对 XOS 的选择性发酵，这些结果进一步表明，B. lactis 和 XOS 的组合将形成一种成功的、选择性的共生组合。
