Caizzi R, Bozzetti M P, Caggese C, Ritossa F
Istituto di Genetica, Università di Bari, Italy.
J Mol Biol. 1990 Mar 5;212(1):17-26. doi: 10.1016/0022-2836(90)90301-2.
We describe the cloning of the glutamine synthetase 1 (GS1) gene based on cross-homology with the glutamine synthetase 2 (GS2) gene in Drosophila melanogaster. We have determined the GS gene number in the Drosophila genome, and we describe the isolation of cDNA clones corresponding to the two isoforms, their entire sequence and their transcription pattern. We looked for subcellular localization of one enzymic isoform; in this way, we were able to locate the GS1 enzyme within the mitochondria of D. melanogaster. We have compared different GS sequences from plants and humans; emerging evolutionary implications are discussed. In addition, we have identified a certain highly stable secondary structure at the nucleotide level in the coding region of isoforms located in the organella.
我们基于与黑腹果蝇谷氨酰胺合成酶2（GS2）基因的交叉同源性描述了谷氨酰胺合成酶1（GS1）基因的克隆。我们确定了果蝇基因组中GS基因的数量，并描述了对应于两种同工型的cDNA克隆的分离、它们的完整序列及其转录模式。我们寻找了一种酶同工型的亚细胞定位；通过这种方式，我们能够将GS1酶定位在黑腹果蝇的线粒体中。我们比较了来自植物和人类的不同GS序列；讨论了新出现的进化意义。此外，我们在细胞器中定位的同工型的编码区核苷酸水平上鉴定出了某种高度稳定的二级结构。
