羽扇豆中的谷氨酰胺合成酶。根瘤特异性cDNA克隆的鉴定与初步表征。
Glutamine synthetase in Lupinus luteus. Identification and preliminary characterization of nodule-specific cDNA clone.
作者信息
Boroń L, Szczygłowski K, Konieczny A, Legocki A B
机构信息
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznaán, Poland.
出版信息
Acta Biochim Pol. 1989;36(3-4):295-301.
PMID:2577229
Abstract
Two glutamine synthetase (GS) cDNA clones from L. luteus were identified and characterized. The nucleotide sequence analysis proved that they represent highly homologous but distinct mRNA species. Northern blot hybridization revealed that pc LINGS encodes the nodule-specific subunit of the GS while pcLIGS1 represents the nonspecific one present in nodule tissue as well as in uninfected roots.
摘要
从藤黄固氮菌中鉴定并表征了两个谷氨酰胺合成酶（GS）cDNA克隆。核苷酸序列分析证明它们代表高度同源但不同的mRNA种类。Northern印迹杂交表明，pcLINGS编码GS的根瘤特异性亚基，而pcLIGS1代表根瘤组织以及未感染根中存在的非特异性亚基。
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