Lebiedzińska A, Da̧browska M, Szefer P, Marszałł M
Department of Food Sciences, Medical University of Gdańsk, Al. Gen. J. Hallera 107, Gdańsk 80-416, Poland.
Toxicol Mech Methods. 2008 Jul;18(6):463-467. doi: 10.1080/15376510701623870. Epub 2008 Jun 23.
Reversed-phase high-performance liquid chromatography, coupled with coulometric electrochemical detection, was successfully applied for the quantification of added folic acid (FA) in fortified fruit juices and cereal products. The method allowed good separation of the 5-HCO-H(4) folate and folic acid in cereal samples. The retention times of vitamins were repeatedly determined by isocratic elution using 40 mM sodium phosphate dibasic, heptahydrate buffer, and 8% acetonitrile (v/v) (0.9 mL/min, pH 5.5) as mobile phase with the Supelco LC 18 column 5 mum (25 cm x 4.6 mm). Folate concentrations were measured using a trienzyme (hog kidney folate conjugase, alpha-amylase, and protease) folate extraction method.
反相高效液相色谱法结合库仑电化学检测法，已成功应用于强化果汁和谷物产品中添加叶酸（FA）的定量分析。该方法能够很好地分离谷物样品中的5-甲酰四氢叶酸和叶酸。使用40 mM磷酸氢二钠七水合物缓冲液和8%乙腈（v/v）（0.9 mL/min，pH 5.5）作为流动相，在Supelco LC 18 5μm（25 cm×4.6 mm）柱上通过等度洗脱反复测定维生素的保留时间。叶酸浓度采用三酶（猪肾叶酸结合酶、α-淀粉酶和蛋白酶）叶酸提取法进行测定。
