Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan.
Inflamm Res. 2010 Feb;59(2):123-7. doi: 10.1007/s00011-009-0078-7. Epub 2009 Aug 21.
Recent studies have demonstrated that a variety of chemokine receptors are expressed in mast cells. We investigated the changes in mRNA expression of CXCRs in murine IL-3-dependent bone marrow-derived mast cells (BMMCs) to clarify how the CXCR expression is regulated in mast cells.
Expression of CXCR mRNA was measured by RNase protection assay. Functional expression of CXCRs was confirmed by monitoring intracellular Ca(2+) mobilization.
CXCR4 mRNA expression was transiently induced in BMMCs in serum-dependent fashion and was completely suppressed upon IgE-mediated antigen stimulation. In contrast, CXCR5 mRNA expression was induced upon IgE-mediated antigen stimulation. Changes in the intracellular Ca(2+) mobilization induced by CXCL12 strongly indicated the functional expression of CXCR4. The decrease in CXCR4 and the increase in CXCR5 mRNA expression was also observed in BMMCs stimulated with thapsigargin, a phorbol ester, and stem cell factor.
The mRNA expression of CXCR4 is differentially regulated in BMMCs upon various stimuli including IgE-mediated antigen stimulation.
最近的研究表明，多种趋化因子受体在肥大细胞中表达。我们研究了鼠白细胞介素 3 依赖性骨髓来源的肥大细胞（BMMC）中 CXCR 的 mRNA 表达变化，以阐明趋化因子受体在肥大细胞中的表达是如何调节的。
通过 RNase 保护测定法测量 CXCR mRNA 的表达。通过监测细胞内 Ca(2+)动员来确认 CXCR 的功能表达。
BMMC 在血清依赖性方式下，CXCR4 mRNA 表达短暂诱导，并且在 IgE 介导的抗原刺激下完全抑制。相比之下，CXCR5 mRNA 表达在 IgE 介导的抗原刺激下诱导。CXCL12 诱导的细胞内 Ca(2+)动员的变化强烈表明 CXCR4 的功能表达。在 BMMC 中也观察到 CXCR4 mRNA 表达的减少和 CXCR5 mRNA 表达的增加，这些 BMMC 受到 thapsigargin（佛波醇酯）和干细胞因子的刺激。
BMMC 中的 CXCR4 mRNA 表达在各种刺激下（包括 IgE 介导的抗原刺激）差异调节。
