Aly Hamdy A A, Lightfoot David A, El-Shemy Hany A
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Al-Azhar University, Nasr City, Cairo, Egypt.
Chem Biol Interact. 2009 Dec 10;182(2-3):112-8. doi: 10.1016/j.cbi.2009.08.013. Epub 2009 Aug 21.
Inflammatory reactions to microbial infections may cause male infertility. The mechanisms of inhibition of spermatogenesis can be studied in vitro using rat Sertoli cells. Bacterial lipopolysaccharides (LPS) induce acute inflammations. So LPS treated Sertoli cells can be used to test for new therapeutic compounds. The present study aimed to investigate the protective efficacy of dl-alpha-lipoic acid (LA) on lipopolysaccharide (LPS)-induced oxidative stress in adult rat Sertoli cells. Sertoli cells were divided into 4 groups. Group I served as a control incubated with water (vehicle). Groups II and IV were incubated with 100 microM LA for 24h before incubating Groups III and IV with 50 microg/ml lipopolysaccharide (LPS) for 12h. In Group III cells (LPS-treated, no LA) the lactate concentration was decreased whereas hydrogen peroxide production and lipid peroxidation were significantly increased. Moreover, the activities of antioxidant enzymes such as superoxide dismutase, glutathione peroxidase, catalase, glutathione-S-transferase, glutathione reductase were reduced. The concentrations of antioxidant molecules such as reduced glutathione and vitamin C were significantly decreased. The activities of enzymes normally elevated in Sertoli cells, gamma-glutamyl transpeptidase and beta-glucuronidase, were significantly decreased. Treatment with LA (100 microM) for 24h before LPS-treatment (Group IV), prevented these changes in enzyme activities and metabolite concentrations. Therefore, LA may have a cyto-protective role during LPS-induced inflammation in adult rat Sertoli cells.
对微生物感染的炎症反应可能导致男性不育。可以使用大鼠支持细胞在体外研究抑制精子发生的机制。细菌脂多糖（LPS）可引发急性炎症。因此，经LPS处理的支持细胞可用于测试新的治疗化合物。本研究旨在探讨dl-α-硫辛酸（LA）对脂多糖（LPS）诱导的成年大鼠支持细胞氧化应激的保护作用。支持细胞分为4组。第I组作为对照组，用水（赋形剂）孵育。第II组和第IV组在孵育前用100 microM LA孵育24小时，然后第III组和第IV组用50微克/毫升脂多糖（LPS）孵育12小时。在第III组细胞（LPS处理，无LA）中，乳酸浓度降低，而过氧化氢产生和脂质过氧化显著增加。此外，超氧化物歧化酶、谷胱甘肽过氧化物酶、过氧化氢酶、谷胱甘肽-S-转移酶、谷胱甘肽还原酶等抗氧化酶的活性降低。还原型谷胱甘肽和维生素C等抗氧化分子的浓度显著降低。支持细胞中通常升高的γ-谷氨酰转肽酶和β-葡萄糖醛酸酶的活性显著降低。在LPS处理前（第IV组）用LA（100 microM）处理24小时，可防止酶活性和代谢物浓度的这些变化。因此，LA在LPS诱导的成年大鼠支持细胞炎症过程中可能具有细胞保护作用。
