Shimoda Kei, Kondo Yoko, Akagi Masaaki, Abe Koichi, Hamada Hatsuyuki, Hamada Hiroki
Department of Pharmacology and Therapeutics, Faculty of Medicine, Oita University, 1-1 Hasama-machi, Oita 879-5593, Japan.
Phytochemistry. 2007 Nov;68(21):2678-83. doi: 10.1016/j.phytochem.2007.05.036. Epub 2007 Jul 5.
Cultured suspension cells of Eucalyptus perriniana converted exogenously administered alpha-tocopherol into alpha-tocopheryl 6-O-beta-d-glucopyranoside (46mug/gfr. wt of cells) and two biotransformation products: alpha-tocopheryl 6-O-(6-O-beta-d-glucopyranosyl)-beta-d-glucopyranoside (19mug/gfr. wt of cells) and alpha-tocopheryl 6-O-(6-O-alpha-l-rhamnopyranosyl)-beta-d-glucopyranoside (6mug/gfr. wt of cells). On the other hand, two other compounds, i.e., delta-tocopheryl 6-O-(6-O-beta-d-glucopyranosyl)-beta-d-glucopyranoside (27mug/g fr. wt of cells) and delta-tocopheryl 6-O-(6-O-alpha-l-rhamnopyranosyl)-beta-d-glucopyranoside (12mug/g fr. wt of cells), together with delta-tocopheryl 6-O-beta-d-glucopyranoside (63mug/g fr. wt of cells) were isolated from suspension cells following the administration of delta-tocopherol.
培氏桉培养的悬浮细胞将外源施用的α-生育酚转化为α-生育酚6-O-β-D-吡喃葡萄糖苷（46μg/克鲜重细胞）以及两种生物转化产物：α-生育酚6-O-（6-O-β-D-吡喃葡萄糖基）-β-D-吡喃葡萄糖苷（19μg/克鲜重细胞）和α-生育酚6-O-（6-O-α-L-鼠李糖基）-β-D-吡喃葡萄糖苷（6μg/克鲜重细胞）。另一方面，在施用δ-生育酚后，从悬浮细胞中分离出另外两种化合物，即δ-生育酚6-O-（6-O-β-D-吡喃葡萄糖基）-β-D-吡喃葡萄糖苷（27μg/克鲜重细胞）和δ-生育酚6-O-（6-O-α-L-鼠李糖基）-β-D-吡喃葡萄糖苷（12μg/克鲜重细胞），以及δ-生育酚6-O-β-D-吡喃葡萄糖苷（63μg/克鲜重细胞）。
