佩里桉培养细胞对生育酚的糖基化作用。

Glycosylation of tocopherols by cultured cells of Eucalyptus perriniana.

作者信息

Shimoda Kei, Kondo Yoko, Akagi Masaaki, Abe Koichi, Hamada Hatsuyuki, Hamada Hiroki

机构信息

Department of Pharmacology and Therapeutics, Faculty of Medicine, Oita University, 1-1 Hasama-machi, Oita 879-5593, Japan.

出版信息

Phytochemistry. 2007 Nov;68(21):2678-83. doi: 10.1016/j.phytochem.2007.05.036. Epub 2007 Jul 5.

DOI:10.1016/j.phytochem.2007.05.036

PMID:17618657

Abstract

Cultured suspension cells of Eucalyptus perriniana converted exogenously administered alpha-tocopherol into alpha-tocopheryl 6-O-beta-d-glucopyranoside (46mug/gfr. wt of cells) and two biotransformation products: alpha-tocopheryl 6-O-(6-O-beta-d-glucopyranosyl)-beta-d-glucopyranoside (19mug/gfr. wt of cells) and alpha-tocopheryl 6-O-(6-O-alpha-l-rhamnopyranosyl)-beta-d-glucopyranoside (6mug/gfr. wt of cells). On the other hand, two other compounds, i.e., delta-tocopheryl 6-O-(6-O-beta-d-glucopyranosyl)-beta-d-glucopyranoside (27mug/g fr. wt of cells) and delta-tocopheryl 6-O-(6-O-alpha-l-rhamnopyranosyl)-beta-d-glucopyranoside (12mug/g fr. wt of cells), together with delta-tocopheryl 6-O-beta-d-glucopyranoside (63mug/g fr. wt of cells) were isolated from suspension cells following the administration of delta-tocopherol.

摘要

培氏桉培养的悬浮细胞将外源施用的α-生育酚转化为α-生育酚6-O-β-D-吡喃葡萄糖苷（46μg/克鲜重细胞）以及两种生物转化产物：α-生育酚6-O-（6-O-β-D-吡喃葡萄糖基）-β-D-吡喃葡萄糖苷（19μg/克鲜重细胞）和α-生育酚6-O-（6-O-α-L-鼠李糖基）-β-D-吡喃葡萄糖苷（6μg/克鲜重细胞）。另一方面，在施用δ-生育酚后，从悬浮细胞中分离出另外两种化合物，即δ-生育酚6-O-（6-O-β-D-吡喃葡萄糖基）-β-D-吡喃葡萄糖苷（27μg/克鲜重细胞）和δ-生育酚6-O-（6-O-α-L-鼠李糖基）-β-D-吡喃葡萄糖苷（12μg/克鲜重细胞），以及δ-生育酚6-O-β-D-吡喃葡萄糖苷（63μg/克鲜重细胞）。