桉树细胞培养物对大豆苷元的糖基化作用。
Glycosylation of daidzein by the Eucalyptus cell cultures.
作者信息
Shimoda Kei, Sato Noriaki, Kobayashi Tatsunari, Hamada Hatsuyuki, Hamada Hiroki
机构信息
Department of Pharmacology and Therapeutics, Faculty of Medicine, Oita University, 1-1 Hasama-machi, Oita 879-5593, Japan.
出版信息
Phytochemistry. 2008 Sep;69(12):2303-6. doi: 10.1016/j.phytochem.2008.05.024. Epub 2008 Jul 5.
The sequential glycosylation of a soybean isoflavone, daidzein, with cultured suspension cells of Eucalyptus perriniana and cyclodextrin glucanotransferase was studied. Daidzein was converted into two glycosylation products, daidzein 7-O-beta-D-glucopyranoside (39%) and daidzein 7-O-[6-O-(beta-D-glucopyranosyl)]-beta-D-glucopyranoside (beta-gentiobioside, 6%), by cultured E. perriniana cells. Further glycosylation of daidzein 7-O-beta-glucoside with cyclodextrin glucanotransferase gave daidzein 7-O-[4-O-(alpha-D-glucopyranosyl)]-beta-D-glucopyranoside (beta-maltoside, 26%), daidzein 7-O-beta-maltotrioside (15%), and daidzein 7-O-beta-maltotetraoside (7%).
研究了大豆异黄酮大豆苷元与佩氏桉培养悬浮细胞和环糊精葡糖基转移酶的顺序糖基化反应。大豆苷元被佩氏桉培养细胞转化为两种糖基化产物，大豆苷元7 - O - β - D - 吡喃葡萄糖苷（39%）和大豆苷元7 - O - [6 - O - (β - D - 吡喃葡萄糖基)] - β - D - 吡喃葡萄糖苷（β - 龙胆二糖苷，6%）。大豆苷元7 - O - β - 葡萄糖苷与环糊精葡糖基转移酶进一步糖基化反应生成大豆苷元7 - O - [4 - O - (α - D - 吡喃葡萄糖基)] - β - D - 吡喃葡萄糖苷（β - 麦芽糖苷，26%）、大豆苷元7 - O - β - 麦芽三糖苷（15%）和大豆苷元7 - O - β - 麦芽四糖苷（7%）。
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