Division of Pharmacognosy, Section Metabolomics, Institute of Biology, Leiden University, Einsteinweg 55, 2333, CC, Leiden, The Netherlands.
Biotechnol Lett. 2009 Dec;31(12):1967-74. doi: 10.1007/s10529-009-0107-1. Epub 2009 Aug 22.
The effect of salicylic acid (SA) on the metabolic profile of Catharanthus roseus suspension cells throughout a time course (0, 6, 12, 24, 48 and 72 h after treatment) was investigated using NMR spectroscopy and multivariate data analysis. When compared to control cell lines, SA-treated cells showed a high level of sugars (glucose and sucrose) up to 48 h after treatment, followed by a dynamic change in amino acids, phenylpropanoids, and tryptamine. Additionally, one compound--2,5-dihydroxybenzoic-5-O-glucoside--was detected solely in SA-treated cells.
采用核磁共振波谱法和多元数据分析研究了水杨酸（SA）对长春花悬浮细胞代谢谱的影响，时间过程为（处理后 0、6、12、24、48 和 72 小时）。与对照细胞系相比，SA 处理的细胞在处理后 48 小时内表现出高水平的糖（葡萄糖和蔗糖），随后氨基酸、苯丙素和色胺发生动态变化。此外，仅在 SA 处理的细胞中检测到一种化合物-2,5-二羟基苯甲酸-5-O-葡萄糖苷。
