Mustafa Natali Rianika
Natural Products Laboratory, Institute of Biology Leiden, Leiden University, Leiden, The Netherlands.
Methods Mol Biol. 2025;2895:137-152. doi: 10.1007/978-1-0716-4350-1_10.
Salicylic acid is a member of benzoic acid derivatives, a group of compounds which have a backbone of C6C1 consisting of one carboxyl group and one (or more) hydroxyl group(s) attached to the aromatic ring. Salicylic acid is a signaling compound in systemic acquired resistance (SAR). An increased level of salicylic acid is found in the plant after a fungi's attack, which further induces the accumulation of phytoalexins, low molecular weight defense compounds. Here, we describe the extraction method and determination of salicylic acid using high-performance liquid chromatography (HPLC) coupled with fluorescence detection.
水杨酸是苯甲酸衍生物的一种，这类化合物具有由一个羧基和一个（或多个）连接在芳环上的羟基组成的C6C1主链。水杨酸是系统获得性抗性（SAR）中的一种信号化合物。植物在受到真菌攻击后，水杨酸水平会升高，这进一步诱导植保素（低分子量防御化合物）的积累。在此，我们描述了使用高效液相色谱（HPLC）结合荧光检测来提取和测定水杨酸的方法。
